Constant-pressure transmembrane clamp for hybridization of Southern imprinting

Abstract

The invention relates to a constant-pressure transmembrane clamp for hybridization of Southern imprinting. The constant-pressure transmembrane clamp comprises a variable-pressure solution storage tank 1, wherein a pressure gauge 2 is fixedly arranged at the bottom of the variable-pressure solution storage tank 1, an objective table 3 is fixedly arranged on the surface of the pressure gauge 2, a clamping plate 4 is arranged above the objective table 3 and can move up and down, holes are formed in two ends of the clamping plate 4, the clamping plate 4 with the holes and the variable-pressure solution storage tank 1 are in sliding connection with each other through lengthened screw rods 6 with detachable rotating nuts 5, the lengthened screw rods 6 with the nuts are fixedly arranged at two sides of the solution storage tank 1, the middle position of the lower part of the clamping plate 4 with the holes is fixedly connected with a hydraulic telescopic rod 8 with a bolt 7, the lower end of the hydraulic telescopic rod 8 is fixedly connected with a pressure plate 9, and a graduated scale 10 is parallel to the screw rod 6 and is fixedly connected with the rear side of the solution storage tank 1. The digitally accurately determined pressure acting on gel can be realized through the pressure gauge arranged at the bottom of the solution storage tank, so as to keep the pressure in the transmembrane process stable. In addition, the fast and convenient replacement of water-absorbing paper under constant-pressure conditions can also be realized, and the optimal transmembrane state can be achieved.

Description

technical field [0001] The invention relates to the technical field of transfer membrane devices, and more specifically, the invention relates to a constant pressure transfer membrane clip for Southern blot hybridization. Background technique [0002] Southern blot hybridization (Southern blot) was created by the British southerner in 1975. It is a basic technique for studying DNA maps and is of great value in the diagnosis of genetic diseases, DNA map analysis and PCR product analysis. Southern blot hybridization is a classic experimental method in molecular biology. Its basic principle is to immobilize the DNA sample to be detected on a solid-phase carrier, hybridize with a labeled nucleic acid probe, and perform a hybridization on the solid-phase DNA with a homologous sequence to the probe. The hybridization signal is displayed at the position. Through Southern hybridization, it can be judged whether there is a fragment homologous to the probe and the length of the fragme...

AI Technical Summary

Problems solved by technology

Although the device improves the stability of the membrane transfer process, the device can only estimate the pressure of the required weight according to the thickness of the gel, and cannot digitally and accurately adjust the pressure acting on the gel, and the device cannot achieve Rapid replacement of absorbent paper under constant pressure will not achieve the best film transfer effect

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