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Method for identifying species of female flesh fly, and application thereof

A technology of female and scorpion flies, which is applied in the field of insect identification, can solve the problems of difficult identification of female scorpion flies, etc., and achieve the effect of short time consumption, high accuracy and widening the scope of use

Active Publication Date: 2014-11-05
岳巧云
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Problems solved by technology

However, the current identification of the species of Sarbina flies internationally is based on the morphology of the tail organs of male Sarbina flies. Different types of female Sarbina flies have little difference in external morphology and have similarities. type

Method used

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  • Method for identifying species of female flesh fly, and application thereof
  • Method for identifying species of female flesh fly, and application thereof
  • Method for identifying species of female flesh fly, and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0052] (1) The mating male and female flies are collected in the wild;

[0053] (2) Use the tail organs and external characteristics of the males to identify the males, so as to determine the species of the paired female flies.

[0054](3) Using DNA barcoding technology, compare whether the DNA barcode sequences of the paired male and female flies are consistent, so as to determine whether the female flies are consistent with the paired male species. The specific steps are as follows: take a leg of the sargassum fly, soak it in the lysate in the animal tissue genomic DNA extraction kit (TIANGEN; code: DP304) for more than 5 hours, and extract the genomic DNA with the animal tissue genomic DNA extraction kit. Using LCO1490 and HCO2198 as primers, by PCR, the reaction system was: 10 μl of 5-fold Phusion HF buffer, 1 μl of 10 mM dNTPs, 1 μl of forward and reverse primers (concentration: 10 pmol / L), 0.5 μl of Phusion ultra-fidelity DNA polymerase, Genomic DNA 2μl, make up 50μl wi...

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Abstract

The invention discloses a method for identifying the species of a female flesh fly, and an application thereof. The method utilizes a uterus spicule to identity the species of the female flesh fly. The inventors of the invention find that the uterus spicule has species specificity, and can be used for identifying the species of the female flesh fly. The method for identifying the species of the female flesh fly through the uterus spicule has the advantages of simplicity, short time and high accuracy. The method is especially suitable for application in the forensic identification field and / or the inspection and quarantine field.

Description

technical field [0001] The invention belongs to the field of insect identification, and in particular relates to a method and application for identifying female sargassum flies. Background technique [0002] There are many kinds of hemp flies, which are distributed all over the world. There are about 2,500 known species in the world. They can carry a variety of pathogens and are one of the important vector organisms. [0003] Accurate identification of the species of sargassum is of great significance for further taking appropriate epidemic prevention measures and preventing the spread of diseases. Since some flies are carnivorous, they are also important forensic insects. According to the developmental state of the flies on the corpse, the time of death and whether it is the first crime scene can be inferred. However, the current identification of the species of Sarbina flies internationally is based on the morphology of the tail organs of male Sarbina flies. Different typ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/48
Inventor 岳巧云汪小东邱德义陈健魏晓雅胡佳刘德星
Owner 岳巧云
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