A kind of preparation method of Luo Han Guo extract suitable for industrial production

Abstract

A preparation method of Luo Han Guo extract suitable for industrial production, comprising the following steps: (1) washing and crushing fresh Luo Han Guo fruit; (2) adding a hydrophilic solvent, heating, refluxing extraction, and filtering to obtain an extract; (3) ) heating the extract, concentrating in vacuum, cooling, standing still, and filtering to obtain the concentrate; (4) subjecting the concentrate to tube centrifugation and ultrafiltration to obtain the filtrate; (5) using ≥3 macroporous resin layers for the filtrate The analysis column is connected in series to the column for adsorption, standing still, washing with water, and then eluted with a hydrophilic solvent and collecting the eluent separately; (6) The eluate is subjected to nanofiltration treatment with a nanofiltration membrane, and then concentrated to 100% About 25-30 to get the concentrate; (7) Spray-dry the concentrate to get the product. The method of the invention can realize one-time production of mogroside V extract products of various specifications, efficiently utilize resin, reduce energy consumption, ensure product quality, reduce cost, and is suitable for industrial production.

Description

technical field [0001] The invention relates to a preparation method of Luo Han Guo extract, in particular to a preparation method of Luo Han Guo extract suitable for industrial production. Background technique [0002] Luo Han Guo, Latin name CorvenorMomordicafruit, also known as Lahan Guo and False Bitter Gourd, is a plant of the genus Cucurbitaceae. It is distributed in Guangxi, Guangdong, Hunan and other provinces in southern China. It is mainly produced in the mountainous areas of Lingui County and Yongfu County in Guilin City. Luo Han Guo contains glycosides, polysaccharides, flavonoids, proteins and amino acids, as well as vitamin C and mineral elements. As a medicinal and edible plant, Luo Han Guo can be used as medicine and edible. Its dried fruit is often used as traditional Chinese medicine, which has good effects of clearing away heat, relieving heat, moistening lung and relieving cough. In addition, Luo Han Guo can be used as a food additive in various desserts,...

AI Technical Summary

Problems solved by technology

[0003] Since Luo Han Guo is a unique plant species in my country and has obvious geographical advantages, the research on the preparation of Luo Han Guo extracts is mainly concentrated in China, and there are many related patent literature reports, but few of them can be really applied to production, mainly reflected in the following aspects : (1) The process is cumbersome and the yield is low; (2) A large amount of organic solvent is used, which requires high equipment and large investment; (3) The process consumes a lot of energy and costs; (4) The introduction of other substances in the process affects the product quality

For example, CN101029071B discloses the method for preparing high-purity mogroside V from Luo Han Guo. The method is to carry out alcohol extraction, concentration, extraction with ethyl acetate and n-butanol to the dried fruit of Luo Han Guo, and then use silica gel column, C18 reverse phase silica gel column chromatography 1. After drying, high-purity sweet glycoside V products are obtained. The main defects of this method are cumbersome process, large solvent loss and low yield, which is not conducive to large-scale industrial production; CN100572552C discloses mogroside V ≥ 40% Luo Han Guo extract and its preparation The method is to extract the fresh fruit of Luo Han Guo with water, concentrate, enzymolyze, absorb it with a macroporous resin, decolorize it with an ion exchange resin, and finally concentrate and dry it to obtain the product. Because this method does not filter before loading the column, the subsequent processing cost of the resin is Larger, not conducive to continuous production; CN101407535B discloses a preparation method of high-purity mogroside V, the method is to use a certain purity of Luo Han Guo extract as raw material, dissolved in methanol, acetone extraction, normal phase silica gel adsorption, separation , crystallization and recrystallization to obtain the final product. This method has a simple process, but a large amount of organic solvents are used in the process, and the requirements for the production site are relatively high. In addition, the obtained products also have certain organic solvent residues, which affect the quality; CN101863946B discloses a A method for extracting high-content mogroside V from dried grosvenor fruit. The method is to crush the dried grosvenor fruit, carry out alcohol extraction, concentration, centrifugation, macroporous resin adsorption separation, ion exchange resin decolorization, and finally concentration and drying to obtain a finished product. Because this method is not filtered, there are more macromolecular impurities in the upper column liquid, which affects the adsorption performance of the resin, the utilization rate is low, the follow-up treatment is relatively difficult, and the product cost is high.