A kind of calla lily test-tube plantlet multiplication method

A technology for test-tube seedlings and calla lilies, which is applied in horticultural methods, botanical equipment and methods, horticulture, etc., can solve the problems of long time from seed sowing to growing into seedlings, inability to carry out industrial production, low reproduction coefficient, etc. The sterile seedlings are neat, the speed of factory production is accelerated, and the cultivation environment is uniform.

Active Publication Date: 2016-06-15
ZHENJIANG AGRI SCI INST JIANGSU HILLY AREAS
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  • Summary
  • Abstract
  • Description
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  • Application Information

AI Technical Summary

Problems solved by technology

[0011] However, when the traditional method is used for reproduction, the reproduction coefficient is very low, the cycle is long, and limited by environmental factors such as climate seasons, factory production cannot be carried out, and the time from seed sowing to growing into seedlings is relatively long

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment

[0019] Embodiment: choose the calla lily test-tube seedlings with consistent growth, and remove the roots for subsequent use.

[0020] Determination of hormone concentration: MS was used as the basic medium for the medium, and 6‐BA (0.6, 1.2, 2.4, 4.8) mg / L, KT (0.6, 1.2, 2.4, 4.8) mg / L, TDZ (0.6, 1.2, 2.4, 4.8) mg / L, sucrose concentration 30g / L, the liquid medium of pH=5.8, every kind of treatment inoculates 20, repeats 3 times, counts the number of lateral buds and the length of lateral buds after 2 weeks of proliferation.

[0021] Determination of the culture method: liquid-liquid culture: culture in liquid MS proliferation medium for 2 weeks, then transfer to hormone-free liquid MS medium and culture for 2 weeks, count the number of lateral buds and the length of lateral buds; liquid-solid culture: liquid MS proliferation culture After 2 weeks of culture in medium, transfer to hormone-free solid MS medium for 2 weeks and count the number and length of lateral buds; solid-s...

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PUM

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Abstract

The invention discloses an alocasia macrorrhizos test-tube plantlet multiplication method which involves MS culture media, a hormone, a carbon source, a cultivation mode and cultivation time. According to the method, alocasia macrorrhizos test-tube plantlets are used as a material, the induction and multiplication effect is obvious, the growth coefficient reaches more than eight, and the alocasia macrorrhizos industrialized production speed can be greatly increased. By the adoption of the method, the problems that a traditional alocasia macrorrhizos division propagation mode is low in propagation coefficient and long in period and can be limited by the environmental factors such as seasons are solved, a liquid cultivation mode is adopted, agar or carrageenan does not need to be added, the tissue culture operation process is reduced, the tissue culture cost can be lowered, the cultivation environment is uniform, nutrient uptake can be promoted, and alocasia macrorrhizos seedlings generated in the liquid cultivation mode are more orderly than aseptic seedlings generated through callus tissue.

Description

Technical field: [0001] The invention belongs to the field of biotechnology (plant tissue culture technology) in agricultural production, and in particular relates to a calla lily test-tube plantlet multiplication system. Background technique: [0002] Calla lily (scientific name: Alocasiamacrorrhizos), called aunt taro in Taiwan, native to South America, is a common ornamental plant with many common names, such as taro scar, chamaejasma (Guangdong), wild taro, mountain taro, big root taro , Dachongyu, Tianyu, and Tianmeng are called dripping Guanyin when they are used as ornamental plants. This is because if the ambient humidity is too high, water will drip from its wide leaves. There is a large green spathe that unfolds into a boat shape, just like the statue of Guanyin. Calla lily is a perennial herb with broad leaves. In northern China, it needs to survive the winter indoors. In the south, it can grow in the open. The bulbs and leaves can be used as medicine, but they a...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01H4/00
Inventor 孙国胜潘跃平马志虎戴忠良毛忠良孙春青吴国平王建华潘永飞张振超秦文斌姚悦梅
Owner ZHENJIANG AGRI SCI INST JIANGSU HILLY AREAS
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