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Alocasia macrorrhizos test-tube plantlet multiplication method

A technology for test-tube seedlings and calla lilies, applied in horticultural methods, botany equipment and methods, horticulture, etc., can solve the problems of long time from seed sowing to seedling growth, inability to carry out factory production, low reproduction coefficient, etc., and achieve The effects of neat sterile vaccines, accelerated factory production, and uniform culture environment

Active Publication Date: 2014-09-10
ZHENJIANG AGRI SCI INST JIANGSU HILLY AREAS
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0011] However, when the traditional method is used for reproduction, the reproduction coefficient is very low, the cycle is long, and limited by environm

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment

[0019] Embodiment: choose the calla lily test-tube seedlings with consistent growth, and remove the roots for subsequent use.

[0020] Determination of hormone concentration: MS was used as the basic medium for the medium, and 6‐BA (0.6, 1.2, 2.4, 4.8) mg / L, KT (0.6, 1.2, 2.4, 4.8) mg / L, TDZ (0.6, 1.2, 2.4, 4.8) mg / L, sucrose concentration 30g / L, the liquid medium of pH=5.8, every kind of treatment inoculates 20, repeats 3 times, counts the number of lateral buds and the length of lateral buds after 2 weeks of proliferation.

[0021] Determination of the culture method: liquid-liquid culture: culture in liquid MS proliferation medium for 2 weeks, then transfer to hormone-free liquid MS medium and culture for 2 weeks, count the number of lateral buds and the length of lateral buds; liquid-solid culture: liquid MS proliferation culture After 2 weeks of culture in medium, transfer to hormone-free solid MS medium for 2 weeks and count the number and length of lateral buds; solid-s...

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PUM

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Abstract

The invention discloses an alocasia macrorrhizos test-tube plantlet multiplication method which involves MS culture media, a hormone, a carbon source, a cultivation mode and cultivation time. According to the method, alocasia macrorrhizos test-tube plantlets are used as a material, the induction and multiplication effect is obvious, the growth coefficient reaches more than eight, and the alocasia macrorrhizos industrialized production speed can be greatly increased. By the adoption of the method, the problems that a traditional alocasia macrorrhizos division propagation mode is low in propagation coefficient and long in period and can be limited by the environmental factors such as seasons are solved, a liquid cultivation mode is adopted, agar or carrageenan does not need to be added, the tissue culture operation process is reduced, the tissue culture cost can be lowered, the cultivation environment is uniform, nutrient uptake can be promoted, and alocasia macrorrhizos seedlings generated in the liquid cultivation mode are more orderly than aseptic seedlings generated through callus tissue.

Description

Technical field: [0001] The invention belongs to the field of biotechnology (plant tissue culture technology) in agricultural production, and in particular relates to a calla lily test-tube plantlet multiplication system. Background technique: [0002] Alocasia macrorrhizos (scientific name: Alocasia macrorrhizos), called aunt taro in Taiwan, is a common ornamental plant native to South America. Taro, chrysanthemum taro, taro, and Tianmeng are called dripping Guanyin when they are used as ornamental plants. This is because if the ambient humidity is too high, water will drip from its large leaves, and its flowers are spadix. Outside there is a large green spathe, which develops into a boat shape, just like the statue of Guanyin. Calla lily is a perennial herb with broad leaves. In northern China, it needs to survive the winter indoors. In the south, it can grow in the open. The bulbs and leaves can be used as medicine, but they are poisonous, and the leaf juice will be pois...

Claims

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Application Information

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IPC IPC(8): A01H4/00
Inventor 孙国胜潘跃平马志虎戴忠良毛忠良孙春青吴国平王建华潘永飞张振超秦文斌姚悦梅
Owner ZHENJIANG AGRI SCI INST JIANGSU HILLY AREAS
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