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Method for collecting blood of submandibular vein of mouse

A blood collection and venous blood vessel technology, applied in medical science, sensors, diagnostic recording/measurement, etc., can solve problems such as eye damage, difficult to stop bleeding, and animals cannot be reused. Effect

Inactive Publication Date: 2014-06-04
WUXI APPTEC SUZHOU +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Among them, blood collection from the heart and abdominal aorta requires anesthesia, and animals cannot be reused; while blood collection from the tail vein is relatively small; blood collection from the orbital sinus requires anesthesia, and it is easy to cause eye damage and is not easy to stop bleeding

Method used

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  • Method for collecting blood of submandibular vein of mouse

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Effect test

Embodiment Construction

[0016] The method for mouse submandibular vein blood collection of the present invention, the steps are as follows:

[0017] 1) prepare

[0018] Check the number of the blood collection tube, prepare Goldenrod Lancet or disposable blood collection needle, clean gauze, cotton ball or paper towel.

[0019] 2) Baoding mice

[0020] Check the animal number, and hold the mouse by the skin on the back of the neck to fix the mouse for restraint. At the same time, during the process of keeping the mice in place, it is necessary to observe the respiratory conditions and mental state of the mice at all times.

[0021] 3) Exposing the blood collection site of the submandibular vein

[0022] Find the lowermost part of the most distal part of the mouse's mandible, and the needle insertion site for blood collection is just behind and above it (such as figure 1 shown).

[0023] If it is difficult to find the vein, it can be rubbed with alcohol (70-75% volume concentration) or hot water ...

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Abstract

The invention discloses a method for collecting blood of a submandibular vein of a mouse. The method comprises the steps of fixing the mouse, exposing the blood collection portion of the submandibular vein, puncturing the skin and a blood vessel bundle of the submandibular vein, collecting the blood and stopping bleeding. The method for collecting the blood has the advantages that operation is easy, injury is small, anesthesia is not needed and the blood can be repeatedly collected and stopped easily, has wide application value and promotes the development of medical research.

Description

technical field [0001] The invention relates to a method for collecting blood in mice, in particular to a method for collecting blood in the submandibular vein of mice. Background technique [0002] In the field of biomedicine, mice are currently the most widely used and most demanded mammalian experimental animals. In many experiments, the blood collection methods of mice are mostly based on the heart, abdominal aorta, tail vein, and orbital sinus. Among them, blood collection from the heart and abdominal aorta requires anesthesia, and animals cannot be reused; while blood collection from the tail vein is relatively small; blood collection from the orbital sinus requires anesthesia, and it is easy to cause eye damage and is difficult to stop bleeding. [0003] Therefore, it is necessary to develop a mouse blood collection method with the advantages of repeatable blood collection, simple operation, and less damage. Contents of the invention [0004] The technical problem...

Claims

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Application Information

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IPC IPC(8): A61B5/153
Inventor 胡璇石同花张伟军薛斌王福强毕玲玲潘华青程妍
Owner WUXI APPTEC SUZHOU
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