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Biomarkers vwf and adamts13 and their use in diagnostic reagents for liver cirrhosis

A diagnostic kit and kit technology, applied in biological testing, disease diagnosis, biological material analysis, etc., can solve the problem of patients being unable to perform FibroScan examination, and achieve safe, non-invasive and easy operation, simple and easy operation, specificity and sensitivity. high sex effect

Active Publication Date: 2016-04-20
重庆早柒天生物科技股份有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

A considerable number of patients cannot be examined by FibroScan

Method used

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  • Biomarkers vwf and adamts13 and their use in diagnostic reagents for liver cirrhosis
  • Biomarkers vwf and adamts13 and their use in diagnostic reagents for liver cirrhosis
  • Biomarkers vwf and adamts13 and their use in diagnostic reagents for liver cirrhosis

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0060] Example 1: Preparation of gold standard method kit and its application in the diagnosis of liver cirrhosis.

[0061] 1. Preparation of the kit:

[0062] The antibody used in the preparation of this kit is the specific antibody of VWF or ADAMTS13. Note that during the preparation of the same kit: if the monoclonal antibody 1 used in the gold-labeled antibody glass fiber membrane is an antibody against VWF, the monoclonal antibody 2 and polyclonal antibody used in the preparation of the corresponding nitrocellulose coating should also use VWF. Antibodies; if the monoclonal antibody 1 used in the gold-labeled antibody glass fiber membrane is an antibody against ADAMTS13, the monoclonal antibody 2 and polyclonal antibody used in the preparation of the nitrocellulose coating should also be an antibody against ADAMTS13.

[0063] 1. Preparation of colloidal gold solution: put ultrapure water on a magnetic stirrer and stir and heat until boiling, quickly add 1% chloroauric aci...

Embodiment 2

[0084] Example 2: Sensitivity and specificity studies of VWF and ADAMTS13 as biomarkers for cirrhosis.

[0085] To define the sensitivity and specificity described here:

[0086] Sensitivity: The sensitivity described here is the proportion of patients with chronic liver disease who actually have cirrhosis that are correctly identified as true positives; or the proportion of patients who actually enter the decompensated stage of cirrhosis as true positives.

[0087] Specificity: The specificity described in this article is the proportion of patients with chronic liver disease who do not actually have cirrhosis as true negatives;

[0088] The critical value of the ratio of VWF and ADAMTS13 in liver cirrhosis: a data ratio of the content of VWF and ADAMTS13 specified in this article, the ratio of the content of VWF and ADAMTS13 in the serum of the subject is higher than this data, it is determined that the patient with chronic liver disease has liver cirrhosis.

[0089] Cirrhos...

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Abstract

The invention relates to the field of use of a biomarker for a disease, and discloses biomarkers von willebrand Factor (VWF) and ADAMTS13 and use thereof in a cirrhosis diagnosis reagent. The VWF disclosed by the invention is formed by protein of which the accession number of an amino acid sequence in a database for the national center of biotechnology information (NCBI) is GI317373549; the ADAMTS13 is formed by protein of which the accession number in the database for the NCBI is GI74749836. A testee is subjected to cirrhosis diagnosis by the biomarkers VWF and ADAMTS13, and the biomarkers have the advantages of being simple and feasible, safe and free of a wound in the diagnosis process, easy to be accepted by a patient, small in effect on diagnostic criteria unification caused by personal subjective factors, high in specificity and sensitivity on diagnosis result, easy to carry out a lot of screening for selection, and the like.

Description

technical field [0001] The present invention relates to the field of use of disease biomarkers, in particular to liver cirrhosis biomarkers VWF and ADAMTS13 and their use in liver cirrhosis diagnostic reagents. Background technique [0002] Cirrhosis is a chronic progressive liver disease characterized by diffuse liver damage caused by long-term or repeated effects of one or more etiologies. In my country, most of them are post-hepatitis cirrhosis, and a few are alcoholic cirrhosis and schistosomiasis. Histopathologically, there are extensive necrosis of hepatocytes, nodular regeneration of residual hepatocytes, hyperplasia of connective tissue and formation of fibrous septa, resulting in the destruction of hepatic lobular structure and the formation of pseudolobules, and the liver gradually deforms and hardens and develops into cirrhosis. In the early stage, there may be no obvious symptoms due to the strong compensatory function of the liver. In the later stage, liver fun...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N33/68G01N33/573G01N33/558
CPCG01N33/543G01N33/558G01N33/6812G01N33/6893G01N2800/085
Inventor 高平罗兰
Owner 重庆早柒天生物科技股份有限公司
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