Litchi R2R3-MYB gene LcMYB1 and application thereof
A R2R3-MYB, gene technology, applied in application, genetic engineering, plant genetic improvement and other directions
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[0032] 1. Litchi peel selection
[0033] The lychee invented separately is selected from the "Nuomici" lychee, and the skin, pulp, leaves, and stems of the "Nuomici" lychee used are taken from the Changgang Mountain Orchard of South China Agricultural University. The developmental stage samples of ‘Nuomici’ were collected at 52 days, 59 days, 62 days, 66 days and 73 days after flowering. Use a 0.9cm caliber puncher to take out the peel discs, put them into a tin foil bag and quickly freeze them with liquid nitrogen, take them back to the laboratory and store them in a -80°C refrigerator for determination of anthocyanin content in the peel, gene cloning and gene expression analyze. ‘Nuomici’ lychee peel, pulp, leaves, and stems were collected from the Changgang Mountain Orchard of South China Agricultural University.
[0034] 2. Extraction and detection of RNA from litchi peel
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