Flow Controlled Chromatography Columns

Abstract

The invention provides a controllable-flow-rate chromatographic column, belonging to the field of biological purification instruments. According to the chromatographic column provided by the invention, the top end of the column body is provided with a U-shaped screen, so that a buffer solution, a sample solution, an eluate and other liquids can conveniently flow down along the wall of the column body, thereby avoiding splashing and influencing the chromatographic effect; the lower part of the liquid outlet is provided with a flow rate controller capable of controlling the flow rate, the flow rate can be directly read out so as to facilitate the experimental operation, and the flow rate is quantified in the chromatographic operate, thereby being beneficial to providing references for subsequent identical or similar protein purification operation; and the column head and column tail are detachable from the column body, so the chromatographic column can be assembled before chromatography, and can be detached after chromatography, and thus, the column head, column tail and column body can be respectively cleaned, sterilized and the like. The chromatographic column has the advantages of simple structure and favorable protein purification effect, and is convenient to operate.

Description

technical field [0001] The invention belongs to biological purification equipment, in particular to a chromatographic column with controllable flow rate. Background technique [0002] At present, the separation and purification of various compound compositions, natural products, and traditional Chinese medicines are mainly achieved by column chromatography, using a chromatography column, and through a column chromatography process. In traditional column chromatography, the mixed compound is placed on the top of the glass column bed, so that the eluent (mobile phase) composed of the mixed solvent passes through a vertical glass column equipped with an adsorptive medium material (stationary phase) under the action of gravity, Separate and obtain each relatively pure product (pure product), its principle is: because each component in the mixed compound has different affinities to the stationary phase and mobile phase in the chromatographic column, so select the appropriate stat...

AI Technical Summary

Problems solved by technology

[0003] The current chromatographic column has the following problems: 1. The flow rate of the liquid cannot be controlled during the chromatographic operation. If the flow rate of the sample liquid is too fast, it is easy to cause the contact time of the target protein and the filler in the column to be too short to be adsorbed. If If the flow rate of the eluent is too fast, it is not easy to exchange and wash the target protein; if the flow rate is too slow, it will increase the chromatography time. Since the flow rate has not been quantified, it cannot provide a reference for the next time the same or similar protein is processed. ; 2. When injecting samples, the sample solution flows directly into the column from the middle of the top of the chromatography column, which may easily cause the filler in the column to splash onto the inner wall of the chromatography column

Since the packing material used for chromatography is often relatively expensive, the packing material splashed on the inner wall is not fully utilized in the chromatography, which affects the chromatographic effect

Images