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Method for improving production intensity of 2-keto-L-gulonic acid (2-KLG) by adding betaine

A technology of ketogenic coulonic acid bacteria and production strength, which is applied in the directions of microorganism-based methods, biochemical equipment and methods, microorganisms, etc., to achieve the effects of suitable proportions, improved production strength, and small pH fluctuations

Inactive Publication Date: 2014-03-19
DSM JIANGSHAN PHARMACEUTICAL (JIANGSU) CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there is no literature report to increase the production intensity of 2-keto-L-gulonic acid by adding betaine

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] Put 6ml of the cultured common ketogenic cologne and Bacillus megaterium mixture into 40ml containing 8% sorbose, 0.5% corn steep liquor, 1.2% urea, 0.02% magnesium sulfate, 0.05% diphosphate Fermentation medium of potassium hydrogen, 0.5% calcium carbonate, and 0.1% betaine, the initial pH of the medium is 7.0, the culture temperature is 29°C, the volume of the shaker flask is 500ml, the rotation speed of the shaker is 210rpm, and the end point 2-KLG content of the fermentation broth is 79.16mg / ml , the fermentation period is 60 hours, and the production intensity is 1.32g / l·h. Compared with the control group, the fermentation period of the experimental group was shortened by 9.1%, and the yield and production intensity of 2-KLG increased by 2.87% and 14.2%, respectively.

Embodiment 2

[0028] Put 12ml of the cultured common ketogenic cologne and Bacillus megaterium into 80ml containing 8% sorbose, 0.5% corn steep liquor, 1.2% urea, 0.02% magnesium sulfate, 0.05% diphosphate Potassium Hydrogen. Fermentation medium with 0.5% calcium carbonate and 0.5% betaine, the initial pH of the medium is 7.0, the culture temperature is 31°C, the volume of the shaker flask is 750ml, the rotation speed of the shaker is 210rpm, the end point 2-KLG content of the fermentation broth is 79.72mg / ml, the fermentation cycle After 56 hours, the production intensity was 1.42g / l·h. Compared with the control group, the fermentation period of the experimental group was shortened by 15.2%, and the yield and production intensity of 2-KLG increased by 2.96% and 16.4%, respectively.

Embodiment 3

[0030] Put 12ml of the cultured common ketogenic cologne and Bacillus megaterium into 80ml containing 8% sorbose, 0.5% corn steep liquor, 1.2% urea, 0.02% magnesium sulfate, 0.05% diphosphate Potassium Hydrogen. Fermentation medium with 0.5% calcium carbonate and 0.2% betaine, the initial pH of the medium is 7.0, the culture temperature is 31°C, the volume of the shaker flask is 750ml, and the rotation speed of the shaker is 210rpm. After 12 hours of fermentation, add 0.02% betaine every 4 hours to the end. The 2-KLG content at the end point of the fermentation broth was 79.95mg / ml, the fermentation period was 54 hours, and the production intensity was 1.48g / l·h. 4.29% and 19.1%.

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Abstract

The invention discloses a method for improving the production intensity of 2-keto-L-gulonic acid (2-KLG) by adding betaine, belonging to the technical field of vitamin C preparation through fermentation. A mixed bacteria system of ketogulonigenium vulgare and bacillus megaterium is adopted as a production strain, and 0.05-1.0% betaine is exogenously added into a fermentation medium as an osmotic pressure regulator so as to promote the cell growth and 2-KLG production and realize efficient production of 2-KLG. By adopting the process, the method disclosed by the invention can effectively shorten the fermentation period of 2-KLG and improve the production intensity.

Description

technical field [0001] The invention belongs to the technical field of vitamin C preparation by fermentation, and more specifically relates to a method for increasing the production intensity of 2-keto-L-gulonic acid by adding betaine. Background technique [0002] Vitamin C is an essential water-soluble vitamin that is involved in various metabolic activities. 2-keto-L-gulonic acid (2-keto-L-gulonic acid, hereinafter referred to as 2-KLG) is an important precursor for the synthesis of vitamin C. At present, major vitamin C manufacturers in China adopt the "two-step fermentation" production process. The key step of this technology is the second fermentation process. L-sorbose is biologically oxidized to 2- KLG, the mixed bacteria system is composed of two kinds of microorganisms, among which the microorganisms responsible for the conversion of sugar and acid are common ketogenic cologne bacteria, commonly known as small bacteria. The other microorganisms in the mixed strai...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P39/00C12P7/60C12R1/11
Inventor 孔太胡少斌陈红刘杰
Owner DSM JIANGSHAN PHARMACEUTICAL (JIANGSU) CO LTD
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