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Processing method of cow urine iTRAQ test data

A technology of data and urine, applied in the direction of measuring devices, instruments, scientific instruments, etc., can solve the problem that the sensitivity and accuracy are difficult to achieve the ideal effect

Inactive Publication Date: 2013-06-19
SHANGHAI CLUSTER BIOTECH
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Problems solved by technology

However, it is difficult to achieve ideal results in terms of sensitivity and accuracy by using traditional methods to measure relative and absolute concentrations of proteins.

Method used

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  • Processing method of cow urine iTRAQ test data

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Embodiment Construction

[0014] According to the present invention, a method for analyzing iTRAQ data is applied to the analysis of protein iTRAQ analysis data. In the present invention, the iTRAQ analysis data of cow urine will be used as an example to illustrate the specific implementation steps of the method:

[0015] Step 1. Differential protein screening. The raw data of the screening comes from the analysis results of the proteinpilot software after iTRAQ detection of dairy cow urine. The data were grouped by experiment and screened between groups to obtain differentially expressed proteins.

[0016] Step 2: GO (Gene ontology) analysis. The differentially expressed proteins obtained in the previous step can be used as input data for GO analysis, and the differentially expressed proteins are transferred to the Gene ontology database ( www.geneontology.org) to map each node and calculate the number of genes at each node. Differential genes are classified according to biological process.

[001...

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Abstract

Aiming at the characteristics of iTRAQ protein quantitative analysis, a method for analyzing cow urine iTRAQ data is designed. The main process includes: step one, screening differential proteins and obtaining the differentiated result by grouping and screening the original iTRAQ data; step two, performing GO analysis, including obtaining more comprehensive gene function information displayed as a directed acyclic graph through mapping query to the GO database; step three, performing Pathway analysis, which is similar with the GO analysis, including construction of interaction paths between genes through the comparison and query of the KEGG database; and step four, performing gene network analysis, including obtaining a gene interaction network diagram through the integration of three different types of interactions.

Description

technical field [0001] The invention belongs to the field of biotechnology, and relates to the quantitative data analysis of cow urine proteomics. Background technique [0002] iTRAQ technology is a new proteomics quantitative research technology newly developed in recent years. It has the advantages of good quantitative effect and high repeatability. It can perform quantitative analysis on up to four different samples at the same time. Workers often use iTRAQ technology to accelerate the quantitative research of proteins. [0003] In the study of proteomics, only knowing the composition of the protein is not enough to give a final conclusion on the protein, because the concentration of the protein is extremely important to realize its function in the cell, and the change in the concentration of a specific protein , can predict the mutation process of cells. However, it is difficult to achieve ideal results in terms of sensitivity and accuracy by using traditional methods ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/86
Inventor 曾华宗
Owner SHANGHAI CLUSTER BIOTECH
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