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Preparation method of collagen-based sponge wound dressing with self-anti-inflammatory function

A collagen-based sponge and wound dressing technology, applied in the application field of biomedical materials, can solve the problems of loss, unable to maintain synergistic anti-inflammatory effect, etc.

Active Publication Date: 2015-05-20
JIANGNAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, due to the excellent water solubility of polyvinylpyrrolidone, it is easy to dissolve and lose in the body or in the environment where there is interstitial fluid exchange, and cannot maintain the synergistic anti-inflammatory effect

Method used

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  • Preparation method of collagen-based sponge wound dressing with self-anti-inflammatory function
  • Preparation method of collagen-based sponge wound dressing with self-anti-inflammatory function
  • Preparation method of collagen-based sponge wound dressing with self-anti-inflammatory function

Examples

Experimental program
Comparison scheme
Effect test

example 1

[0011] Example one (using liquid cross-linking method)

[0012] First, add 10g of polyvinylpyrrolidone to 100mL of potassium hydroxide solution with a concentration of 50mg / mL, then react in a pressure-resistant sealed bottle at a temperature of 130°C for 12 hours, remove the solvent, reconstitute with dichloromethane, and filter out the insoluble After the solvent was evaporated to dryness, the ring-opened polyvinylpyrrolidone was obtained, and the ring-opening rate was 4%. Mix 100mg of collagen with 100mg of ring-opened polyvinylpyrrolidone and dissolve to a concentration of 10mg / mL, then add 1mmol of 1-(3-dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride and 0.5 1 mmol of N-hydroxysuccinimide was activated and cross-linked in the mold for 16 hours at a cross-linking temperature of 25°C. Afterwards, the cross-linked product was soaked and rinsed with deionized water to remove excess activator and freeze-dried to obtain a sponge product , and the samples were stored in ...

example 2

[0013] Example two (using solid state cross-linking method)

[0014] First, add 2.5 g of polyvinylpyrrolidone to 100 mL of potassium hydroxide solution with a concentration of 25 mg / mL, then react in a pressure-resistant sealed bottle at a temperature of 140 °C for 24 hours, remove the solvent, redissolve with dichloromethane, and filter out After the insoluble matter was evaporated to dryness, the ring-opened polyvinylpyrrolidone was obtained, and the ring-opening rate was 8%. Mix 100mg collagen with 50mg ring-opened polyvinylpyrrolidone and dissolve it to a concentration of 7.5mg / mL, freeze-dry it in a mold, and then soak the freeze-dried product in a solution containing 1-(3-dimethylaminopropyl)-3 - the concentration of ethylcarbodiimide hydrochloride is 50mmol / L, the concentration of N-hydroxysuccinimide is 25mmol / L in 90% acetone solution to activate cross-linking, the cross-linking time is 24 hours, the cross-linking temperature temperature at 20°C, and then soak and ri...

example 3

[0016] The physical and chemical properties of the prepared composite sponge dressing were evaluated, and the pure collagen sponge was used as the blank control.

[0017] 1. Experimental method

[0018] (1) Determination of swelling rate:

[0019] Weigh 10 mg of completely dry sponge sample and soak it in PBS buffer (pH7.4), at the specified time point, take out the sample, carefully wipe off the surface solution, and then weigh (W t ), the weight after fully swelling is W s . The swelling rate of the sponge (Swelling ratio, SR) is calculated according to the following formula:

[0020] SR(%)=(W t -10)×100 / W s

[0021] (2) Determination of maximum water absorption and water retention rate:

[0022] First soak 10 mg of the fully dried sponge sample in PBS buffer (pH7.4) for 24 hours to swell completely, and the weight is W s , the maximum water absorption is W s -10, then put it into a centrifuge tube with a filter, centrifuge at 500rpm for 3 minutes, then take it out ...

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Abstract

The invention belongs to the field of applications of biomedical materials and specifically relates to a preparation method of a collagen-based sponge wound dressing with a self-anti-inflammatory function. The dressing is of a sponge type, is soft in texture, has a uniform porous structure, and can be used for fast absorbing water for swelling, effectively reducing the secretion of inflammatory factors, eliminating inflammation of a wound, absorbing secretions of the wound and promoting the repair of the wound; and simultaneously, the sponge is resistant to enzymatic degradation, can be used for reducing the number of times of replacement and can be applied to repair of a variety of skin wounds, such as burns and mechanical injuries. The preparation method comprises the following steps: performing partial ring opening on polyvinylpyrrolidone in a potassium hydroxide solution at high temperature and high pressure, further mixing the polyvinylpyrrolidone after ring opening with collagen to prepare a solution, taking 1-(3-dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride and N-hydroxysuccinimide as activators for cross-linking, then performing deionized water soaking and washing on a cross-linked product, removing the surplus activators, and then freeze-drying for getting the dressing.

Description

technical field [0001] The invention relates to a preparation method of a collagen-based sponge wound dressing with self-anti-inflammatory function. It belongs to the application field of biomedical materials. Background technique [0002] Inflammation is a common pathological reaction after cortical injury, which can cause redness, swelling, fever, and pain in the wound. Large-area and deep cortical injuries will stimulate the body to produce severe inflammatory reactions, resulting in edema, ulceration, and even necrosis of wounded tissues, making it difficult for wounds to heal, and the large amount of toxins produced will also damage body functions. Therefore, people have been looking for ways to reduce inflammation. Methods to promote wound repair. Wound dressings are commonly used medical hygiene materials, which can protect wounds, prevent infection, and promote wound repair. However, traditional gauze dressings cannot promote wound repair and may cause secondary d...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C08J3/24C08J9/28C08L39/06C08L89/00A61L15/32A61L15/24A61L15/44
Inventor 陈荆晓宋加毅邓超陈敬华
Owner JIANGNAN UNIV
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