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Question mark dependent type leptospira replicator loss ratio analysis method

A leptospira and gene-replicating technology, applied in the field of microorganisms, can solve the problems affecting whether the replicating gene produces new functions or remains silent and eliminated, and achieves the effect of simple use and convenient operation

Active Publication Date: 2013-05-15
TONGLING RUIPU PEONY IND DEV
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Problems solved by technology

The difference in the selection constraints will affect whether the replicator gene will produce new functions or remain silent, or even be eliminated

Method used

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  • Question mark dependent type leptospira replicator loss ratio analysis method
  • Question mark dependent type leptospira replicator loss ratio analysis method
  • Question mark dependent type leptospira replicator loss ratio analysis method

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Embodiment Construction

[0026] The technical solution of the present invention will be described in detail below in conjunction with specific embodiments of the accompanying drawings.

[0027] The principle of the present invention is as follows: firstly, by comparing Leptospira interrogans and picking out the duplicated genes, and then calculating the substitution numbers of each synonymous site and each non-synonymous site of each pair of duplicated genes respectively , and finally including constructing differential equations using properties and using regression analysis to obtain relevant parameters to understand the changes in selection constraints during the evolution of replicators, and to introduce the concept of S as the divergence time to infer the loss rate of replicators.

[0028] refer to figure 1 , a kind of Leptospira question mark Lai type gene loss rate analysis method, comprises the following steps:

[0029] 1) Obtain the replicating gene of Leptospira interrogans

[0030] Leptos...

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Abstract

The invention discloses a question mark dependent type leptospira replicator loss ratio analysis method. Firstly, comparison jetting-out replicator is conducted on a question mark dependent type leptospira, then replacing number of each synonymy point and each non-synonymy point of each pair of replicators is respectively calculated, and finally function fitting is conducted by using of property structure differential equation and regression analysis. Change condition of selection of constraining force is understood in the replicator evolutionary process, the concept that S is used as divergence time is introduced, and copy loss rate of the replicators is deduced.

Description

technical field [0001] The invention belongs to the technical field of microbes, and relates to a method for analyzing the loss rate of replication genes of Leptospira interrogans, in particular to a method for analyzing the loss rate of replication genes of Leptospira interrogans based on differential equations and regression analysis. Background technique [0002] Question mark Lai type Leptospira (Leptospira interrogans serovarLai) is a kind of Leptospira (Laptospira), Leptospira is an ancient microorganism, belongs to a branch of fungi in evolution. Leptospirosis is a worldwide zoonotic disease. The main symptoms include fever, muscle pain, and fatigue. In severe cases, it is found to be extensive bleeding of organs, and kidney and lung function damage. The Leptospira interrogans that we study is a representative and highly toxic Leptospira. Its biological characteristics and pathogenicity are unique, and it is also one of the two epidemic strains in my country. Leptosp...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G06F19/10
Inventor 王晓峰王参军王洪柯苏盈盈孙宝光
Owner TONGLING RUIPU PEONY IND DEV
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