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Device for sequentially dispensing liquid reagents to a reaction chamber

A sequential, reactive technique, applied in the field of appliances or systems, capable of solving the problems of delays in the diagnosis and treatment of human and animal diseases, the problem of undesired tests, etc.

Inactive Publication Date: 2013-04-10
AVACTA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] However, this causes delays and is especially undesirable for assays used in the diagnosis and treatment of human and animal diseases

Method used

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  • Device for sequentially dispensing liquid reagents to a reaction chamber
  • Device for sequentially dispensing liquid reagents to a reaction chamber
  • Device for sequentially dispensing liquid reagents to a reaction chamber

Examples

Experimental program
Comparison scheme
Effect test

example 1

[0094] Example 1 Cartridge Preparation

[0095] Assemble the cartridge using the assembly procedure below:

[0096] First wipe the reagent and wash tubes with silicone oil to lubricate the tubes. Four reagent plugs and two wash solution plugs were then inserted into the respective tubes using set length plugs to create three reagent aliquot spaces and one wash solution space within the respective tubes. Appropriate volumes of desired reagents and washes are dispensed through the fill holes on the reagent and wash tubes into the aliquot spaces and fill hole caps are inserted into the fill holes to seal them. Insert the cap of the waste tube into the end of the waste tube to seal.

[0097] An adhesive (such as a liquid, foam, gel, or tape-type adhesive) is applied to the area surrounding the fluid channel on the test cartridge and the slide with the prepared functionalized reaction area placed on the onto the fluid channels and presses onto the applied adhesive so that it b...

example 2

[0098] Example 2 Assay system

[0099] Cartridges prepared in Experiment 1 as described above can be used in conjunction with a reading unit to perform automated ELISA tests.

[0100] The test cartridge is first filled with reagents, washes, and sample, and the slide portion of the cartridge is functionalized with biomolecules (proteins, antibodies, antigens, nucleotides) specific to the sample analyte of interest. After inserting the cartridge into the reading unit, the user selects the appropriate pre-programmed test and chooses to start the test. The reading unit then drives the test reagent and wash fluid through the cartridge by pushing the reagent partition spacer and the wash fluid spacer according to a predetermined program. As the reagents and washes are sequentially passed through the biomolecule functionalized area on the slide, the desired assay occurs due to the appropriate incubation period of the reagents on the functionalized reaction area.

[0101] Next, E...

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PUM

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Abstract

An apparatus for delivering a plurality of liquid volumes sequentially to a reaction site is provided, the apparatus comprising a vessel having a plurality of solid spacers arranged in linear order therein so as to define a series of adjacent voids, separated by individual solid spacers, for receiving said liquid volumes, the vessel being provided in a lateral wall with a plurality of reversibly sealable openings through which the liquid volumes for delivery to the reaction site may be introduced into the voids defined therein, and an outlet port, the spacers being movable within the vessel such that, in use, liquid volumes disposed in the individual voids are deliverable sequentially through the 'outlet port to the reaction site. Also provided are units, such as disposable cartridge units, comprising such an apparatus coupled to a reaction site and systems comprising such units together with means for interrogating the reaction site to determine the outcome of the reaction.

Description

technical field [0001] The present invention relates to apparatus or systems for use in performing biological or chemical reactions, and also to units, especially disposable units, for use in such systems, and methods of using such apparatus and units for assaying biological or chemical samples. Background technique [0002] In many chemical or biological sensing, assay, and diagnostic applications, it is necessary to deliver liquid reagents, solvents, or washes to reaction sites for chemical or biological reactions. [0003] Typically, the cost and complexity of the equipment required to perform such biological and chemical assays, together with the risk of contamination and cross-contamination, means that samples are typically obtained in the field and sent to a laboratory for analysis. [0004] However, this causes delays and is especially undesirable for assays used in the diagnosis and treatment of human and animal diseases. It is therefore worth considering the develo...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): B01L3/00G01N35/10
CPCB01L3/502B01L2200/16B01L2300/0832B01L2300/087B01L2400/0478B01L2400/0481G01N35/1002
Inventor E.J.奎因S.韦布斯特D.A.史密斯R.哈兰德
Owner AVACTA
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