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Microbial fermentation and detoxification method of rapeseed cake

A microbial fermentation and rapeseed cake technology, which is applied in the field of detoxification of rapeseed cake and microbial fermentation detoxification of rapeseed cake, can solve the problems of complex process, poor microbial adaptability, unstable detoxification effect, etc. Achieve the effects of simple production process, easy degradation, and shortened detoxification time

Active Publication Date: 2013-06-19
HUNAN AGRICULTURAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although the detoxification method by microbial fermentation has achieved good results, there are still shortcomings such as unstable detoxification effect, complicated process and poor microbial adaptability.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

preparation example Construction

[0030] Preparation of liquid strains:

[0031] The strains used were 1 strain of Bacillus subtilis, 1 strain of Clostridium butyricum, 1 strain of Lactobacillus acidophilus and 1 strain of Saccharomyces cerevisiae.

[0032] Take 15g of glucose, 8g of peptone, 4g of beef extract, 4g of sodium chloride and 1000mL of tap water, stir well, sterilize at 0.1MPa pressure for 25min, cool to 30°C and insert the cultured mature Bacillus subtilis slant strain, at 35°C, Under 200r / min, the shaker was shaken for 30h to prepare the Bacillus subtilis seed culture solution;

[0033] Take 30g glucose, 18g tryptone, 1.2g ammonium sulfate, 2.2g sodium chloride, 1.2g sodium bicarbonate, 3.5g dipotassium hydrogenphosphate, 0.6g magnesium sulfate heptahydrate, 1.5g calcium carbonate and 1000mL water Sterilize under 0.1MPa pressure for 25 minutes, cool to 30°C, inoculate and culture mature Clostridium butyricum slant strains, and culture at 38°C for 68 hours to prepare Clostridium butyricum seed cu...

Embodiment 1

[0039] Inject the liquid strains into the above-mentioned solid strain culture medium at 8% inoculation weight, cultivate at 37°C for 4.5 days to obtain solid strains, and then inoculate the solid strains with 3% inoculation weight into rapeseed cake 8.25 tons, 1.52 tons of rice bran, 0.07 tons of glucose, (NH 4 ) 2 SO 4 0.15 tons and 0.01 tons of white mustard seeds, in the rapeseed meal detoxification fermentation medium with a water content of 50%, mixed evenly, fermented at 50°C for 4.5 days, and dried to obtain the detoxified rapeseed meal. Sampling and analysis showed that the detoxification rate of glucosinolate in rapeseed cake was 96.13%, the protein content was 43.17%, and the free amino acid content increased by 12.49%.

Embodiment 2

[0041] Add liquid strains to the above solid strain culture medium at 5% inoculation weight, and culture at 35°C for 5 days to obtain solid strains, and then inoculate the solid strains at 2% inoculation weight to rapeseed cake 8.5 tons, 1.25 tons of rice bran, 0.05 tons of glucose, (NH 4 ) 2 SO 4 0.186 tons and 0.014 tons of black mustard seeds, in the rapeseed cake detoxification fermentation medium composed of 55% water content, mixed, fermented at 45°C for 5 days, and dried to obtain detoxified rapeseed cake. Sampling and analysis showed that the detoxification rate of glucosinolate in rapeseed cake was 95.87%, the protein content was 42.9%, and the free amino acid content was increased by 11.52%.

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Abstract

The invention discloses a microbial fermentation and detoxification method of rapeseed cake. The method comprises the following steps: adding a crushed detoxification synergist to the rapeseed cake; inoculating a bacillus subtilis, a clostridia butyricum, a lactobacillus acidophilus and a saccharomyces cerevisiae solid strain to the mixture and mixing evenly; fermenting, drying the mixture to obtain a detoxified rapeseed cake product. According to the invention, the content of glucoside sulphate in the detoxified rapeseed cake product does not exceed a safety level specified by the feed, the nutritional value is higher than that of the unfermented and undetoxified rapeseed cake, the protein content reaches 42 to 45%, and the content of disassociated amino acid reaches more than 10%, so that the detoxified rapeseed cake product can be directly used as protein feed for livestocks and aquatic products. The method provided by the invention can better solve the problems of incomplete detoxification, difficult elimination of anti-nutritional factors, nutrition loss and complexity in preparation process in prior art. The method has the advantages of good detoxification effect, simplicityin preparation and low energy consumption, and can reach the standard feed requirement of producing the non-toxic rapeseed cake. Therefore, the problem of shortage of feed protein resources in China is solved.

Description

technical field [0001] The invention belongs to the technical field of feed biology, and relates to a detoxification method of rapeseed cake, in particular to a microbial fermentation detoxification method of rapeseed cake. Background technique [0002] Rapeseed is one of the most important oil crops in my country, accounting for a quarter of the country's total oil raw materials. Rapeseed is processed under modern industrial conditions, and nearly 60% of the rapeseed meal can usually be obtained. The crude protein content in rapeseed meal is between 34%-38%, digestible energy 10.46-12.55KJ / kg, calcium 0.61%, phosphorus 0.95%, and also contains iron, copper, manganese, selenium and other trace elements and multivitamins . Its amino acid composition is characterized by high methionine content (second only to sesame cake and meal), high lysine content, and low arginine content, which is the lowest content in cake and meal feed. The effective energy value of rapeseed meal is...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/14A23L5/20
Inventor 肖调义兰时乐许宝红毛小伟周芳如李立恒管桂萍
Owner HUNAN AGRICULTURAL UNIV
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