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Selection breeding method of new homozygous sterile line of brassica napus

A Brassica napus homozygous technology, applied in botany equipment and methods, applications, plant gene improvement, etc., can solve the problems of inoperable transgenic materials and heavy workload

Inactive Publication Date: 2013-12-25
贵州省油菜研究所
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] The technical problem to be solved by the present invention is to overcome the defects that the existing methods for breeding new homozygous sterile lines of Brassica napus have a large workload and are almost impossible to operate when there are many transgenic materials, and provide a new homozygous male sterile line of Brassica napus CMS breeding method

Method used

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  • Selection breeding method of new homozygous sterile line of brassica napus
  • Selection breeding method of new homozygous sterile line of brassica napus
  • Selection breeding method of new homozygous sterile line of brassica napus

Examples

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Effect test

Embodiment 1

[0037] Example 1: In the spring of 2005, the pure and sterile line 105A was crossed with 827R, wherein the 105A line is a stable pure and sterile material isolated from the recessive genic male sterile three-line hybrid Wanyou 19, but the high yield of the material Sex, quality and economic traits are poor. 827R is derived from the fertile line in the female parent 8227AB of Youyan No. 10. After segregation and selection, there is no material with stable fertility in segregation of sterile plants. Excellent, especially the oil content is as high as 48% to 51%. The transgenic material was selected in Sinan (Guiyang) and Weining for a total of 7 to 8 planting seasons (Table 2), and a new homozygous sterile line C02A was bred in 2009. Its fertility After several generations of test crossing and hybrid seed production, the combination with the stable Temporary Line all showed the characteristics of complete sterility (Table 3).

example 2

[0038] Example 2: In 2007, the pure and sterile line 105A was crossed with 898×1400, and the 105A line was a stable pure and sterile material isolated from the recessive genetic male sterile three-line hybrid Wanyou 19, but the high yield of the material 898 in 898×1400 comes from the excellent selection line of Zhongshuang 4, and 1400 is an excellent selection line of Huyou 17. This material has high yield, good economic properties, high quality, and resistance Preferably, the oil content is around 45%. The transgenic material was selected for 7-8 planting seasons in Sinan (Guiyang) and Weining (Table 2), and a homozygous male sterile line C04A was bred in 2011. The hybrid combinations of line preservation test cross and hybrid seed production all showed the characteristics of total sterility (Table 3).

[0039] Table 2 Breeding process

[0040]

[0041] Table 3 Fertility identification performance of the cross between homozygous sterile line and temporary insurance lin...

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Abstract

The invention discloses a selection breeding method of a new homozygous sterile line of brassica napus; according to the method, a F2 fertile plant to a transfer material is used for inbreeding; as for F3, a part of the F2 inbred single plants is hybridized with a temporary maintainer line for seed production so as to identify the F2 inbred single plant material with a homozygous sterile genotype; then the homozygous sterile line is separated and selected by the reserved single plant material; and productivity identification is performed on hybrids prepared by a restorer with seeds obtained in hybrid seed production by the selected material and the temporary maintainer line during fertility identification. The method saves a lot of labor force used in plantation and hybridization of the selected colony during selection, can predict the combining ability of the selected material at an early stage, and thus improves the breeding efficiency.

Description

technical field [0001] The invention relates to a method for breeding rapeseed, in particular to a method for selecting a new homozygous sterile line of Brassica napus. Background technique [0002] Rapeseed is the main oilseed crop in my country. Since the first hybrid rapeseed Qinyou No. Ten years later, high-quality rapeseed breeding and heterosis breeding have been successfully combined, and a large number of varieties with high yield, high quality and high oil content have been bred and popularized in China. [0003] In rapeseed heterosis breeding, the application in production is realized mainly by creating and breeding rapeseed male sterile lines. At present, the sterile lines used in a large area mainly include cytoplasmic male sterility and nuclear male sterility, and nuclear male sterility is divided into recessive nuclear male sterility and dominant nuclear male sterility. There are few sources, and there are not many varieties bred. Therefore, except for the thr...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01H1/02A01H1/04
Inventor 侯国佐
Owner 贵州省油菜研究所
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