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Cell culture system

A technology of cell culture and liquid culture medium, applied in the field of cell culture system, can solve problems such as damage to quality, damage to cells or tissues

Inactive Publication Date: 2013-01-02
FRAUNHOFER GESELLSCHAFT ZUR FOERDERUNG DER ANGEWANDTEN FORSCHUNG EV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

This may also result in damage to cells or tissues and / or impair the quality of the final product thus obtained

Method used

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Embodiment Construction

[0051] figure 1 is a schematic cross-sectional view of an insert or container (30) of the present invention comprising a capillary opening (42) in at least one wall element (38, 40) of the container. The container (30) is designed to be housed in the wells (14) of a multiwell plate or similar vessel. The container ( 30 ) is mainly designed as a cylindrical or conical rotationally symmetrical shape and contains side wall elements ( 40 ) and bottom wall elements ( 38 ). The bottom wall element (38) may further comprise a preferably liquid impermeable membrane (39) for allowing the diffusion of nutrients and gases through the bottom of the container (30). In one mode of operation, the container (30) holds a first liquid or gel (61), which is preferably a cell culture composition, such as a cell culture substrate, a cell suspension, or a three-dimensional multilayered tissue construct. The first liquid or gel (61) is preferably covered by a second liquid (62), for example simila...

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Abstract

A microtiter plate based cell culture system comprising an improved cell culture insert which is suitable and used for the cultivation of adherent cells and / or three-dimensional tissues.

Description

technical field [0001] The present invention relates to containers for holding liquids or gels in carriers or vessels, and in particular to cells for being held in microwell plates to form cell culture systems suitable for culturing adherent cells or three-dimensional tissues Cultivate plug-ins (inserts) and their uses. Background technique [0002] Currently, artificial biological tissue constructs, such as artificial skin or artificial cartilage substitutes, are only manufactured in very small batches, and in most cases even only as individual products. Cells required for the manufacture of artificial biological tissues are usually obtained by biopsy. Cells are isolated and placed in a cell culture system for propagation. Currently, conventional cell culture systems consist of so-called multi-well or microtiter plates in which multiple containers or depressions ('wells') are formed in the surface for the parallel cultivation of several cell cultures in one carrier. The ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12M1/12C12M3/06
CPCC12M25/04
Inventor 克里斯蒂安·赖斯安德烈亚斯·特劳贝托拜厄斯·布罗德
Owner FRAUNHOFER GESELLSCHAFT ZUR FOERDERUNG DER ANGEWANDTEN FORSCHUNG EV
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