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Method for rapid nondestructive detection of liver tumor marker and test paper strip adopted by the method

A liver tumor and marker technology, applied in the field of liver tumor marker detection, can solve the problems of complex detection process, inconvenient sampling, infectious diseases, etc., and achieve the effect of accurate detection and easy sample collection.

Inactive Publication Date: 2013-01-02
NORTHWEST UNIV(CN)
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Problems solved by technology

[0008] In summary, it can be expected that if the method of detecting biomarkers in serum samples is adopted, sampling is inconvenient, the detection process is complicated, and there is a risk of infection of some diseases

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  • Method for rapid nondestructive detection of liver tumor marker and test paper strip adopted by the method
  • Method for rapid nondestructive detection of liver tumor marker and test paper strip adopted by the method

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Embodiment Construction

[0042] The difficulty of saliva detection lies in: First, more than 98% of the components in saliva are water, and proteins, salts and some enzyme components only account for a very small proportion. Therefore, it is difficult to identify the proteome in saliva, especially the glycoproteome. Secondly, the biomarker proteins that can be used for detection are often low-abundance proteins, and it is difficult to identify them even in plasma. The use of saliva for detection further increases the difficulty of identification. It often requires researchers to go through a lot of experiments and analysis, and make targeted adjustments in the detection process to obtain effective identification results.

[0043] The present invention also confirms the corresponding saliva N-glycopeptide concentration by carrying out the following experimental method for detecting liver tumor markers (concentrating and changing liquid, enzymatic hydrolysis and desalting, and enriching saliva N-glycop...

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Abstract

The invention provides a method for rapid nondestructive detection of a liver tumor marker and a test paper strip adopted by the method. A gold maker pad of the test paper strip adopted by the method comprises glass fibers adsorbed with colloidal gold-marked monoclonal antibodies GP73-1. A nitrocellulose film is orderly marked with a detection band T1 and a quality control line C, wherein monoclonal antibodies GP73-2 are fixed to the detection band T1 and rabbit anti-mouse polyclonal antibodies Ig are fixed to the quality control line C. The method for rapid nondestructive detection of a liver tumor marker comprises the following steps of taking a saliva sample, inserting an end of a sample pad of the test paper strip which is an immunochromotographic test paper strip into the saliva sample for 10 to 15min so that the saliva sample appears on a NC film, taking out the test paper strip, and observing coloring states of the detection band and the quality control line to obtain a detection result. The method utilizes a saliva sample as a detection object and compares the saliva sample with a serum sample and other body fluid samples. The method has the advantages of easy sample acquisition, nondestructive detection, accurate detection, safety, convenience, low cost and easy preservation.

Description

technical field [0001] The invention relates to a method for detecting liver tumor markers and a detection tool used therefor. Background technique [0002] Primary liver cancer, especially hepatocellular carcinoma (HCC) has been one of the most important cancers in the world for a long time. Its prevalence rate is increasing year by year in our country. The surgical resection rate of the disease is low, and the 5-year survival rate is less than 5%. It is now the second leading cause of cancer death in my country. Liver cancer usually progresses slowly and is asymptomatic in the early stages. Currently, a combination of serum alpha-fetoprotein (AFP) testing and ultrasonography of the liver is the standard method for monitoring and screening for liver cancer. However, these methods have limited sensitivity and specificity. Many studies have shown that the sensitivity of AFP detection is low (only 50-60%) and its diagnostic accuracy is not satisfactory. Ultrasound detectio...

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/68G01N33/577G01N33/558
Inventor 李铮赵菲王秦哲于汉杰张华孙士生
Owner NORTHWEST UNIV(CN)
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