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Bacillus megaterium and application thereof

A technology of Bacillus megaterium and plant growth-promoting bacteria, applied in Bacillus megaterium and its application fields, can solve the problems of the soil environment that may not be suitable for fluvo-aquic soil, achieve good growth-promoting effect, high utilization rate, and promote absorption

Inactive Publication Date: 2013-09-11
鸿坤土壤修复科技(苏州)有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, most of the plant growth-promoting bacteria are still domesticated into bacteria that can be effectively used to promote plant growth and have better nitrogen fixation and phosphorus dissolution effects, and may not be suitable for the soil environment of fluvo-aquic soil.

Method used

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  • Bacillus megaterium and application thereof
  • Bacillus megaterium and application thereof
  • Bacillus megaterium and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0039] First prepare the following three media.

[0040] LB medium: peptone 10g, yeast extract 5g, sodium chloride 10g, agar 20g, distilled water 1000ml, pH7.0-7.2, sterilized at 121°C for 20min.

[0041] LB liquid medium: without agar, other conditions are the same as above.

[0042] Inorganic phosphorus bacteria medium (PKO medium): 5g tricalcium phosphate, 10g glucose, 0.5g ammonium sulfate, 0.3g sodium chloride, 0.3g magnesium sulfate heptahydrate, 0.3g potassium chloride, 0.03g manganese sulfate, heptahydrate Ferrous sulfate 0.03g, pH7.0 agar 20g, distilled water 1000ml, pH7.0~7.2. Sterilize at 121°C for 20min.

[0043] Inorganic salt medium: ammonium sulfate 2.0g; sodium dihydrogen phosphate 0.5g; dipotassium hydrogen phosphate 0.5g; magnesium sulfate heptahydrate 0.2g; calcium chloride dihydrate 0.1g, distilled water 1000mL, pH7.0, sterilized at 121℃ , 20min.

[0044] Weigh 10g of the fluvo-aquic soil collected from Banqiao, Nanjing, and put it in a 250ml Erlenmeyer ...

Embodiment 2

[0052] Aerobic test

[0053] Pour the sterilized LB culture medium into 3 sterilized test tubes, at about 2 / 3, on the aseptic operating table, pick up the JX15 (CGMCC No.5622) cultured on the slant with an inoculation needle, and puncture Inoculate into the above medium (must be pierced to the bottom of the tube). Cultivate at 30°C, and observe the results in 3 days to 7 days respectively. Those that grow on the surface of the agar column are aerobic bacteria, and those that grow along the puncture line are anaerobic or facultative anaerobic bacteria. The test results showed that JX15 (CGMCC No.5622) colonies grew along the surface of the agar column, and no colonies grew in the puncture line, which was strictly aerobic.

[0054] Determination of catalase

[0055] Put 1 drop of 3% H on a clean slide 2 o 2 , take 1 ring of 18~24h LB slant culture, in H 2 o 2 Smear in the middle, if bubbles are produced, it is positive, otherwise it is negative. The test results showed t...

Embodiment 3

[0081] In order to further verify the ability and optimal conditions of the plant growth-promoting bacteria JX15 (CGMCC No.5622) obtained in Example 1 to produce indole acetic acid, the following is to explore the indole acetic acid for different pH, liquid volume, different carbon sources, and different nitrogen sources. Effect on acetic acid production.

[0082] Put 25ml, 50ml, 75ml, 100ml, 150ml of LB liquid medium containing L-tryptophan (100mg / L) into a 250mL Erlenmeyer flask, and inoculate at 1% (v / v) inoculum in logarithmic growth phase After JX15 (CGMCC No.5622), put it at 30℃, 180r·min -1 Cultivate on a shaking table for 24 hours, and measure the amount of IAA produced by a quantitative method. The result is as figure 2 As shown, because the strain JX15 (CGMCC No.5622) has a good metabolism, the aeration rate affects the efficiency of the strain to produce IAA. When the liquid volume is 50mL, the strain produces the most IAA, and then as the liquid volume increases...

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Abstract

The invention discloses bacillus megaterium and an application thereof, and belongs to the field of microorganisms. A plant growth promoting bacterium (bacillus megaterium JX15) is collected in the China General Microbiological Culture Collection Center on Dec.20, 2011, the class name is bacillus megaterium and the collection number is CGMCC No.5622. The bacillus megaterium has the beneficial effects that a strain can produce indoleacetic acid with high yield, can be used for nitrogen fixation and can grow by using sparingly-soluble phosphates as phosphorous sources, so as to improve the utilization rates of fertilizers, promote plant root development and absorption of the fertilizers, and increases the contents of soil mineral nitrogen and available phosphorus; the strain has a good effect on promoting growth of peanuts; the high-yield indoleacetic acid promotes the growth and the development of the peanuts; and the nitrogen and phosphate fertilizer utilization rates of the peanuts are relatively high due to the increasing of the contents of the soil mineral nitrogen and the available phosphorus.

Description

technical field [0001] The invention belongs to the field of agricultural microorganisms, and relates to a bacillus megaterium and an application thereof. Background technique [0002] Fluvo-aquic soil is the soil formed by river sediments affected by groundwater movement and farming activities. It is named after the night tide phenomenon. In China, it is mostly distributed in the alluvial plains of the middle and lower reaches of the Yellow River, the plains of Jiangsu and Anhui to the south, and the river, lake plains and deltas of the middle and lower reaches of the Yangtze River Basin. The distribution area of ​​fluvo-aquic soil is flat, with deep soil layer, abundant water and heat resources, and wide variety of seed production. It is the main dryland soil in my country and is rich in grain and cotton. However, the Huang-Huai-Hai Plain, where the fluvo-aquic soil is the largest, suffers from frequent droughts and floods, as well as saline-alkali hazards. In addition, s...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20A01N63/02A01P21/00C05F11/08A01G1/00A01G7/00C12R1/11
Inventor 李辉信姜瑛徐文思李引陈剑东胡锋焦加国徐莉刘满强陈小云
Owner 鸿坤土壤修复科技(苏州)有限公司
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