Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Method for culturing dental pulp cells and method for transporting extracted tooth for preservation

A technology of dental pulp cells and isolated teeth, which is applied in the field of transport of isolated teeth, and can solve problems such as the pain of the collection object

Inactive Publication Date: 2014-01-08
GENE TECH SCI CO LTD
View PDF7 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, when stem cells are collected from the above-mentioned tissues, the recipient often has to bear risks such as pain. For example, bone marrow needs to be harvested by bone marrow aspiration, and the harvest from fat tissue needs to be accompanied by liposuction.
In addition, although the collection of umbilical cord blood is almost painless, it requires equipment that meets strict collection conditions, and the timing of collection is limited to the time of delivery

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Method for culturing dental pulp cells and method for transporting extracted tooth for preservation
  • Method for culturing dental pulp cells and method for transporting extracted tooth for preservation
  • Method for culturing dental pulp cells and method for transporting extracted tooth for preservation

Examples

Experimental program
Comparison scheme
Effect test

Embodiment

[0064] Examples of the present invention are shown below, but the present invention is not limited to these examples.

[0065] First, an example of the transfer (preservation) of isolated teeth (primary teeth, permanent teeth) according to the method of the present invention will be shown.

[0066]

[0067] deciduous teeth

[0068] According to the dental method, the shaking deciduous teeth without caries and forming more than 2 / 3 of the roots of the successor permanent teeth, or the non-shaking deciduous teeth with caries that have not reached the enamel and only dentine and formed more than 2 / 3 of the roots of the successor permanent teeth. After unplugging, put it into a sterile test tube filled with α-MEM medium, refrigerate at low temperature (4°C), and store for 24 hours including the delivery time.

[0069] permanent teeth

[0070] Extraction of impacted teeth, supernumerary teeth or convenient extraction teeth without caries, untreated pulp according to the dental ...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

Provided are a method for culturing dental pulp cells without compromising the in vivo function of the dental pulp cells, and a method for transporting an extracted tooth for preservation. The method for obtaining dental pulp cells includes: a step for forming a linear groove in the surface of an extracted tooth; a step for dividing the extracted tooth along the groove and exposing the dental pulp; and a step for soaking the extracted tooth in a culture medium and conveying the soaked tooth in a state maintained at a temperature appropriate for cell storage.

Description

[0001] related application [0002] This application claims the priority of Japanese Patent Application No. 2009-289090 filed in Japan on December 21, 2009, and the contents thereof are incorporated into this application. technical field [0003] The present invention relates to a method for transferring an isolated tooth for culturing and preserving dental pulp cells, and particularly relates to improving the preservation of the cells in transferring an isolated tooth having a dental pulp containing dental pulp cells. Background technique [0004] Since ancient times, research and development of regenerative medicine has been active in regenerating tissues or organs lost due to trauma, disease, accident, etc., using collected cells, as is known by blood transfusion. In recent years, the specific process of regenerative medicine has targeted the following process: collecting undifferentiated stem cells, culturing them in vitro, differentiating them depending on the situation...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Patents(China)
IPC IPC(8): C12N5/00C12N5/0775A61L27/00A61L27/38
CPCC12N5/0654A01N1/02A61L27/00C12N5/00C12N5/0602
Inventor 斋藤一郎大久保亮大友宏一
Owner GENE TECH SCI CO LTD
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com