Method for detecting multiple harmful substance residual components in food based on surface plasma resonance technique

Abstract

The invention relates to a method for detecting multiple harmful substance residual components in food based on a surface plasma resonance (SPR) technique. The method comprises four steps of sensing surface aptamer fixing, sample marking, sample detecting and sensing element regenerating. The surface of a multi-probe optical fiber sensor is connected with the aptamer corresponding to different residual components; a molecular probe marker corresponding to different residual components is added and introduced on the sensing surface; unmarked and marked residual components carry out specificity competitive identification on the corresponding aptamer; molecular probe aptamer solution is introduced and combined with the marked sample connected on the surface of the optical fiber sensor for SPR detection by using the multi-probe optical fiber sensor; and the multi-probe optical fiber sensor can be used for regenerating to realize repeated use of the sensing element. Based on the SPR technique and the aptamer specificity competitive identification, multiple harmful substance residual components in one sample can be simultaneously detected, and the method provided by the invention is particularly suitable for detecting substances with small molecular weight.

Description

technical field [0001] The invention relates to a simultaneous detection technology for multiple residues of harmful substances in food. In particular, based on surface plasmon resonance technology, through the specific competitive recognition of nucleic acid aptamers and unlabeled and labeled samples, a multi-probe optical fiber sensor is used to simultaneously detect multiple harmful substances in food. Background technique [0002] Residues of harmful substances in food have seriously endangered public health. Facing the major challenge of food safety, analysis and detection of harmful substances in food is an important part of ensuring food safety. Among them, Surface Plasmon Resonance (SPR), as an ultra-sensitive and rapid detection method, is one of the ideal tools for food safety detection. However, SPR still has the disadvantages of large signal-to-noise ratio, small response value, and low sensitivity when detecting low molecular weight substances, while acetamipri...

AI Technical Summary

Problems solved by technology

[0004] In order to solve the problems existing in the prior art, the present invention provides a method for detecting residues of various low-molecular-weight harmful substances in food using surface plasmon resonance spectroscopy technology, which solves the problem that it is difficult to detect multiple low-molecular-weight harmful substances at the same time in the existing surface plasmon resonance technology. The problem with molecular weight substances

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