Method for improving low germination rate of sterile line of three lines of rice

Abstract

The invention relates to a method for improving the low germination rate of a sterile line of three lines of rice. The method is characterized in that Sanming dominant genic male sterile rice is taken as a carrier, the maintainer line of the low-germination-rate sterile line of three lines is taken as a recurrent parent, a Gm6 gene is transferred into the maintainer line of the low-germination-rate sterile line of three lines through 67 times of back-crossing, a fertile plant containing the homozygous Gm6 gene similar to the original maintainer line is obtained after self-crossing, a pedigree selection method is adopted in the generation-adding and homozygous process of the fertile plant to select a great quantity of individual plants for conducting continuous back-crossing, germination rate screening and parent difference removal by taking the original sterile line of three lines as a cytoplasm donor, and a new sterile line of three lines which is basically consistent with the original sterile line of three lines and satisfies the commercial standard on the germination rate can be obtained finally. The method has the advantages that the germination rate of the improved sterile line of three lines is improved and satisfies the commercial standard, the improved sterile line of three lines contains the Gm6 gene and the genetic constitutions of the improved sterile line of three lines are basically consistent with the genetic constitutions of the original sterile line of three lines.

Description

Technical field: [0001] The invention relates to a method for improving the low germination rate of a three-line sterile line of rice, comprising transducing the rice Gm6 gene into a maintainer line of the three-line sterile line with a low germination rate, screening for germination rate, and eliminating parental differences. Background technique: [0002] The discovery of triple-obvious genital sterility in rice: In 2001, when the fertility identification of the two-line genital sterile line was carried out in Fengyuan Village, Xicheng Town, Youxi County, Fujian Province, more than 800 plants of F 2 In the generation segregation population, a male sterile plant was found to have a different form of pollen abortion from other male sterile plants. The aborted anthers had a special shape, filaments and anthers were small, almost linear, white, and the anthers did not dehisce. After test cross, backcross, and sister crosses, the fertility separation of offspring was investigat...

AI Technical Summary

Problems solved by technology

[0004] The object of the present invention is to overcome the defect that the three-line male sterile line of rice has a low germination rate and provide an improved method for the low germination rate of the three-line male sterile line of rice