Fermented rose extract for cigarettes and preparation method thereof
A rose extract, fermented technology, applied in the application field of microbial fermentation technology, can solve the problems of increasing irritation, application restrictions, increasing residues, etc., and achieve the effects of reducing irritation, softening smoke, and increasing sweetness
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Embodiment 1
[0019] (1), the rose and water are refluxed and extracted at a ratio of 1:5 for 1 hour, filtered through a 0.8 μm microporous membrane, and the filtrate is collected to obtain a rose water extract;
[0020] (2), add the glucose of 0.1% and 0.05% sodium chloride by weight percentage to the rose water extract to prepare the culture medium;
[0021] (3) Inoculate Saccharomycescerevisiae HHL-1 with the preservation number CCTCC NO: M209272 and the preservation name Saccharomycescerevisiae HHL-1 into the LB medium so that the bacterial concentration reaches 1.5×10 10 cerevisiae culture solution; under aseptic conditions, inoculate 0.1% aroma-producing bacteria Saccharomyces cerevisiae HHL-1 solution;
[0022] (4), culture medium is placed in culture fluid and fermented;
[0023] (5), the primary fermentation liquid obtained after fermentation is filtered under a 0.8 μm microporous membrane, the clarified liquid is obtained, and then filtered under a 0.2 μm microporous membrane to ...
Embodiment 2
[0027] (1), the rose and water were refluxed and extracted in a ratio of 1:10 for 3 hours, filtered through a 0.8 μm microporous membrane, and the filtrate was collected to obtain a rose water extract;
[0028] (2), add the glucose of 2% and 0.75% sodium chloride by weight percentage to the rose water extract to prepare the culture medium;
[0029] (3) Inoculate Saccharomycescerevisiae HHL-1 with the preservation number CCTCC NO: M209272 and the preservation name Saccharomycescerevisiae HHL-1 into the LB medium so that the bacterial concentration reaches 1.5×10 10 cerevisiae culture solution; under aseptic conditions, inoculate 0.01% aroma-producing bacteria Saccharomyces cerevisiae HHL-1 solution;
[0030] (4), culture medium is placed in culture fluid and fermented;
[0031] (5), the primary fermentation liquid obtained after fermentation is filtered under a 0.8 μm microporous membrane, the clarified liquid is obtained, and then filtered under a 0.2 μm microporous membrane ...
Embodiment 3
[0035] (1), the rose and water were refluxed and extracted at a ratio of 1:8 for 2 hours, filtered through a 0.8 μm microporous membrane, and the filtrate was collected to obtain a rose water extract;
[0036] (2), add the glucose that is 5% and 0.3% sodium chloride by weight percentage to the rose flower water extract and make culture medium;
[0037] (3) Inoculate Saccharomycescerevisiae HHL-1 with the preservation number CCTCC NO: M209272 and the preservation name Saccharomycescerevisiae HHL-1 into the LB medium so that the bacterial concentration reaches 1.5×10 10 cerevisiae culture solution; under aseptic conditions, inoculate 0.01% aroma-producing bacteria Saccharomyces cerevisiae HHL-1 solution;
[0038] (4), culture medium is placed in culture fluid and fermented;
[0039] (5), the primary fermentation liquid obtained after fermentation is filtered under a 0.8 μm microporous membrane, the clarified liquid is obtained, and then filtered under a 0.2 μm microporous membr...
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