Bioflocculant fermentation method with mycelium pellet as vector

A technology for fermentation organisms and mycelial balls is applied in the field of fermentation biological flocculants, which can solve the problems of being unsuitable for large-scale industrial fermentation production, low production efficiency and high production cost, reducing the chance of contamination by miscellaneous bacteria, improving production efficiency and flocculation. rate stabilization effect

Inactive Publication Date: 2013-03-13
HARBIN INST OF TECH
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  • Application Information

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Problems solved by technology

[0004] The purpose of the present invention is to solve the problems of high energy consumption, low production efficiency, high production cost and unsuitability for large-scale industrial fermentation production in the fermentation method of the existing biological flocculant, and provides a fermentation method using mycelium balls as a carrier. The Bioflocculant Method

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  • Bioflocculant fermentation method with mycelium pellet as vector
  • Bioflocculant fermentation method with mycelium pellet as vector
  • Bioflocculant fermentation method with mycelium pellet as vector

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specific Embodiment approach 1

[0009] Specific embodiment 1: In this embodiment, the method for fermenting biological flocculants with mycelium balls as a carrier is carried out in the following steps: 1. After mixing Rhizobium radiata and Bacillus sphaericus in a volume ratio of 1:1, inoculate it into a 100mL flocculation-producing container In a 250mL Erlenmeyer flask with bacterial culture medium, place it in a shaker with a temperature of 20-30°C and a rotation speed of 120-160r / min for 18-30 hours to obtain the floc-producing fungus seed liquid; 2. The spore suspension of Aspergillus niger Inoculate into a 250mL Erlenmeyer flask containing 100mL of mycelium ball medium, place it in a shaker with a temperature of 28-32°C and a rotation speed of 140r / min and cultivate it for 1-7 days to obtain mature mycelium balls; 3. Mature Rinse mycelium balls with sterilized phosphate solution and add them to a 250mL Erlenmeyer flask containing 100mL flocculogen culture medium, then add flocculogen seed liquid at a vo...

specific Embodiment approach 2

[0015] Specific embodiment 2: The difference between this embodiment and specific embodiment 1 is that in step 1, it is placed in a shaker with a temperature of 20° C. and a rotation speed of 160 r / min for 30 hours to obtain flocculogen seed liquid. Other steps and parameters are the same as those in Embodiment 1.

specific Embodiment approach 3

[0016] Specific embodiment 3: The difference between this embodiment and specific embodiment 1 is that in step 1, it is placed in a shaker with a temperature of 30° C. and a rotation speed of 120 r / min for 18 hours to obtain flocculogen seed liquid. Other steps and parameters are the same as those in Embodiment 1.

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Abstract

The invention relates to a bioflocculant fermentation method, in particular to a bioflocculant fermentation method with a mycelium pellet as a vector. The method solves the problems of high energy consumption, high production cost and unsuitability for large-scale industrialized fermentation production and the like existing in the existing bioflocculant fermentation method and comprises the following steps of: 1, mixing rhizobium radiobacter with bacillus sphaericus and then culturing so as to obtain flocculant-producing bacteria seed liquid; 2, culturing aspergillus niger to be a mature mycelium pellet; 3, mixing the mature mycelium pellet with the flocculant-producing bacteria seed liquid and then culturing so as to obtain a mixed mycelium pellet; 4, taking 24-hour as a fermentation period, and drawing out the mixed mycelium pellet after each fermentation period is finished and pouring the mixed mycelium pellet into fresh culture mediums for culturing; and 5, repetitively operating the step 4 for 30-35 times and namely completing. According to the bioflocculant fermentation method provided by the invention, on the premise of guaranteeing stable flocculating rate, seed liquid demand is reduced, production efficiency is increased and production energy consumption is reduced; moreover, the invention has great significance on large-scale industrialized production of bioflocculant.

Description

technical field [0001] The invention relates to a method for fermenting biological flocculants. Background technique [0002] Bioflocculant (Bioflocculant, BF) is a kind of natural biopolymer produced by microorganisms, which has the functions of flocculation and sedimentation for suspended matter such as bacteria, cells, solid particles, and colloidal particles in liquid. Compared with chemical flocculants, biological flocculants have many advantages, such as high efficiency, non-toxicity, low fermentation cost, no secondary pollution, biodegradable, harmless degradation products, and easy solid-liquid separation of products. Flocculants meet the requirements of sustainable development today, and have important practical significance for human health and environmental protection. It has become one of the important directions of flocculant research and development. It is a new type of green water purifier. [0003] At present, the research on bioflocculants focuses on the i...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12P39/00C12R1/685C12R1/07C12R1/41
Inventor 马放王金娜李昂杨基先王继华邢洁张斯张淑娟
Owner HARBIN INST OF TECH
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