Tissue Culture Rapid Propagation Method of Ginger Lotus

Abstract

The invention discloses a rapid tissue culture and propagation method of curcuma alsimatifolia, belonging to the technical field of plant tissue culture. The method comprises the following steps of: firstly, preparation of a culture medium; secondly, culture and sterilization of earthnut budlet; thirdly, culture of explant sterile seedling; fourthly, induction culture of adventitious bud; fifthly, propagation culture of adventitious bud; sixthly, root growing culture of plantlet; and seventhly, seedling adaptation, acclimation and transplanting of tissue culture seedling. The method disclosedby the invention has the advantages that propagation culture period is short (14-20 days), propagation coefficient is high (improved to 5-8 times from 2-4 times), the consistency of the adventitious bud is good, tissue culture breeding cycle is short (50-60 days), seedling quality is good, and transplanting survival rate can reach up to more than 95%, and is a method for efficiently and rapidly providing high-quality curcuma alsimatifolia seedlings. The method disclosed by the invention can be popularized and applied to landscaping and tissue culture seedling enterprises.

Description

technical field [0001] The invention relates to the technical field of plant tissue culture, in particular to a tissue culture rapid propagation method of the flower plant ginger lotus. Background technique [0002] Ginger lotus (Curcuma alismaifolia Gagnep.) is a perennial tropical herbaceous perennial flower of the genus Zingiberaceae, native to Chiang Mai, Thailand. Because its pink bracts resemble lotus and belong to the ginger family, it is called ginger lotus. Ginger lotus is a spike-like inflorescence, the pedicel is higher than the leaf surface, the flower color is beautiful and bright, and the flowering period is long-lasting. It is a new type of fresh-cut flower variety with high ornamental value, high quality, high economic benefits, and broad prospects for development and utilization. Ginger lotus is favored by people in Japan and Taiwan because of its unique flower shape, bright color and long flowering period. At present, the mainland market is almost blank, a...

AI Technical Summary

Problems solved by technology

But at present, we can only rely on conventional propagation methods, and the propagation speed is very slow, which is far from meeting the market's demand for its seedlings. Therefore, the use of tissue culture rapid propagation technology can obtain high-quality and neat seedlings, and open up an effective way for its rapid propagation

[0003] "Research on Tissue Culture Technology of Ginger Lotus" (Mou Xiaoling, 2006, Northern Horticulture) and "Tissue Culture and Rapid Propagation Technology of New Ginger Lotus Variety Red Guanyin" (Shang Hongli, 2010, Northern Horticulture) have disclosed the tissue culture of Ginger Lotus. cultivation method, but said method still has the following deficiencies: the propagation coefficient of adventitious buds is low, only 2-4 times; The simultaneous growth of adventitious buds and adventitious roots not only seriously affects the multiplication efficiency, but also makes the subculture division of adventitious buds difficult to operate, which eventually leads to a decline in seedling quality and a low transplant survival rate, generally only about 82%.