Preparation method for burkholderia cepacia, and application in high-performance phosphate-dissolving microbial fertilizer by using the same

Abstract

The invention relates to a preparation method for burkholderia cepacia, and an application in high-performance phosphate-dissolving microbial fertilizer by using the same. The method is characterized in that: the method comprises the following steps: (1) collecting deficient phosphorus soil for planting corns, putting the soil in a plastic bag, and placing the plastic bag in a 4 DEG C refrigerator for storing; (2) separating and screening a (burkholderia cepacia) strain; (3) detecting phosphorus solubilizing capability of the (burkholderia cepacia) strain; (4) identifying the strain: wherein the screened strain is subjected to a DNA sequencing and a 16SrRNA phylogenetic tree construction to be identified as the burkholderia cepacia. According the present invention, activation rate of the insoluble phosphate in the soil can reach to 10.94%-13.03%, utilization rate of the P reaches to 21.75%-37.98%, and the phosphorus fertilizer amount can be reduced by one third in application for the corns. The phosphate-dissolving microbial fertilizer provided by the present invention has advantages of low cost, good effect, disease resistance of plants enhancing, no pollution on environment and fully utilization of potential phosphorus resource.

Description

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AI Technical Summary

Benefits of technology

This patented technology helps activating solubility-dispersing elements like calcium from plants effectively without causing excessive damage or harmful chemicals during production processes. It also increases the efficiency of applying microbial fertils with high levels of nitrogen (N) content compared to traditional methods such as adding urea). Additionally, it allows farmers to use less expensive sources of nutrients while still producing effective crops due to its ability to stimulate root growth.

Problems solved by technology

This patent describes various technical problem addressed in this patents relating to improving efficiency or productivity of nitrogen fixated vegetative macrophytes such as legumes and cycling grass roots through decompositing insolvent phosporylite metabolites called phytophyllobiontic acid(PB) residues within their own root systems without causing excessive loss of beneficial ones like phosphorous. Existing methods involve adding other substances to stimulate phosphor release, resulting in decreased yields and reduced activity levels. To address this issue, efforts should focus on finding effective ways to break down insolvability complexities associated with certain mineral nutrients found naturally occurring throughout nature.

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