Application of bdellovibrio bdelloplast in preparing bacterial agent for preventing and controlling red mouth disease of scophthalmus maximus

A technology of Bdellovibrio and turbot, which is applied in the field of application of Bdellovibrio bdelloplasts in the preparation of antibacterial agents for preventing and treating turbot red-mouth disease, can solve problems such as food safety, and achieve effective application, good safety, Apply Promising Effects

Inactive Publication Date: 2012-07-18
SOUTH CHINA UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] In order to overcome the deficiencies of various food safety problems caused by the abuse of antibiotics in fish farming, the present invention provides an application of a Bdellovibrio bdelloplast in the preparation of a bacterial agent for preventing and treating turbot redmouth disease

Method used

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  • Application of bdellovibrio bdelloplast in preparing bacterial agent for preventing and controlling red mouth disease of scophthalmus maximus
  • Application of bdellovibrio bdelloplast in preparing bacterial agent for preventing and controlling red mouth disease of scophthalmus maximus
  • Application of bdellovibrio bdelloplast in preparing bacterial agent for preventing and controlling red mouth disease of scophthalmus maximus

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Embodiment 1

[0020] The preparation of embodiment 1 bacterial agent

[0021] The Bdellovibrio strain used was Bdellovibrio BDM01. The Bdellovibrio sp. BDM01 was preserved in the China Center for Type Culture Collection on April 28, 2008, and the preservation number is CCTCC NO: M 208066; after it was negatively stained, its morphology was observed under an electron microscope: Bdellovibrio sp. The bacteria BDM01 is single-celled, arc-shaped, with a size of 1.7×1.0 μm, terminal flagella, and the length of the flagella is 3.5 μm; it can form a transparent circular phagocytosis with a diameter of 3-4 mm when cultured at 28°C for three days by double-layer plate method. plaque.

[0022] Inoculate 1 mL of hydrophilic aerosol at a concentration of 109 cfu / mL in a conical flask filled with 100 mL of normal nutrient broth (NB) medium (peptone 10 g, beef extract 3 g, sodium chloride 5 g, distilled water 1000 mL, pH 7.4) Aeromonas hydrophila (purchased from Guangdong Provincial Institute of Microb...

Embodiment 2

[0024] Select juvenile fish with healthy physique and uniform individual size, with an average body weight of 55±2.5g, according to 300 fish / m 2 The breeding density is put into the pond, and the experimental period of each breeding pond is 4 months.

Embodiment 21

[0026] The normal running water adopts external drainage, and the amount of changing water every day and night is 8 running water volumes, and the conventional turbot pellet feed (the breeding method produced by the factory) is put in without adding bacterial agents.

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Abstract

The invention discloses application of bdellovibrio bdelloplast in preparing a bacterial agent for preventing and controlling red mouth disease of scophthalmus maximus. The bdellovibrio is (Bdellovibrio sp.) BDM01. The application method for the bacterial agent comprises that: the bacterial agent is put into water body to prevent and control the red mouth disease of scophthalmus maximus to ensurethat the final concentration of the bdellovibrio bdelloplast in the water body is 10-10<7>pfu / mL, or a feed is soaked by the bacterial agent for controlling the red mouth disease of scophthalmus maximus for 15 to 45 minutes, and the concentration of the bdellovibrio bdelloplast in the bacterial agent is 10-10<7>pfu / mL. When the bdellovibrio bdelloplast is applied to preparing the bacterial agent for preventing and controlling the red mouth disease of scophthalmus maximus, the bacterial agent can effectively prevent and control the red mouth disease of scophthalmus maximus, has high safety when applied to aquaculture, is environment-friendly, does not cause pathogenic bacteria resistance, and has good application prospect.

Description

technical field [0001] The invention belongs to the field of biotechnology, and relates to the application of a fungus, in particular to the application of the Bdellovibrio bdelloplast in preparing a fungal agent for preventing and treating turbot red-mouth disease. Background technique [0002] Turbot (Scophthalmus maximus) is a rare and valuable flounder native to Europe. Because it has the advantages of adapting to low water temperature life, fast growth, delicious meat, easy to accept artificial feed, and easy to intensive farming, after being introduced to China in 1992, it quickly became an important cultured species in the northern coast of China. However, the rapid development and non-standard operation of breeding have caused the problem of diseases to become more and more prominent, and many obvious symptoms and large-scale deaths have occurred. Red mouth disease is one of them, which has caused heavy economic losses to farmers. The main symptoms of red mouth dise...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20A61K9/10A61K9/127A61P31/04A61K35/74
Inventor 蔡俊鹏陈丽芸
Owner SOUTH CHINA UNIV OF TECH
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