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Application of high mobility group protein B2 as liver cell carcinoma marker

A high-mobility, hepatocellular carcinoma technology, applied in biological tests, instruments, measurement devices, etc., can solve problems such as the correlation between high-mobility group protein B2 and hepatocellular carcinoma that has not yet been seen

Inactive Publication Date: 2011-03-30
SHANGHAI INST OF BIOLOGICAL SCI CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0008] But so far, there is no report on the correlation between high mobility group box B2 and hepatocellular carcinoma in the prior art

Method used

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  • Application of high mobility group protein B2 as liver cell carcinoma marker
  • Application of high mobility group protein B2 as liver cell carcinoma marker
  • Application of high mobility group protein B2 as liver cell carcinoma marker

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] Example 1 , Preparation of protein samples from hepatocellular carcinoma tissue and paracancerous tissue

[0031] The cancer tissues and paracancerous tissues of 5 patients with hepatocellular carcinoma were obtained from Dongfang Hepatobiliary Surgery Hospital. The 5 patients were all diagnosed by 2 pathologists as suffering from hepatocellular carcinoma. The pathological data of the 5 patients are shown in Table 1. Show.

[0032] Table 1. Pathological data of 5 cases of hepatocellular carcinoma specimens

[0033] No.

gender

age

HBV

HCV

grade

AFP

size

P1

male

58

+

-

III

1000

5.2×6.4

P2

male

44

+

-

III

1000

8×8×7

P3

male

55

+

-

III

1000

4×3

P4

male

40

+

-

III

1000

10×8×6

P5

male

65

+

-

III

1000

11.5×6.5

[0034] Then, non-...

Embodiment 2

[0036] Example 2 , HepG2 and L02 13 Culture and sample preparation of C-labeled cells

[0037] in contain 13 C-labeled CLM-2262L-Leu, 10% dialyzed fetal bovine serum, L-Gln, L-Lys and L-Met were cultured in 6 cm culture dishes of D9785 medium, respectively, to culture HepG2 or L02 cells.

[0038] After the cells had grown to 6 passages, the HepG2 cells or L02 cells were transferred to a 10 cm culture dish for culture.

[0039] When the cell growth density reaches 80%, wash three times with ice-cold PBS, and add lysate to lyse. Then use a spatula to combine the two 13 The cells marked with C were collected by scraping, sonicated in an ice bath, centrifuged at 15,000 g for 1 h, and the supernatant was taken, and the total protein content was quantified by the modified Bradford method (see the product manual of Bio-Rad Company).

Embodiment 3

[0040] Example 3 , Screening of differentially expressed proteins in cancer tissues and paracancerous tissues

[0041] Using CDIT technology (refer to Yasushi Ishihama et al.Nat Biotechnol.2005; 23(5):617-621) combined with in-solution enzymatic hydrolysis technology (refer to William M.Old et al.Mol Cell Proteomics.2005; 4:1487-1502 ) and yin-yang multidimensional liquid chromatography tandem mass spectrometry (refer to Jie Dai et al.J Proteome Res.2007; 6(1):250-262) to screen differentially expressed proteins, the specific process is as follows:

[0042] Get 100ug each of the 5 pairs of hepatocellular carcinoma tissue and paracancerous tissue protein samples obtained in Example 1, respectively mix the protein samples of 5 cases of hepatocellular carcinoma tissue and the paracancerous tissue samples of 5 cases of hepatocellular carcinoma to obtain 500ug of cancer tissue Protein samples and 500ug paracancerous tissue protein samples.

[0043] Take embodiment 2 13 Protein ...

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Abstract

The invention provides an application of high mobility group protein B2, in particular to an application of high mobility group protein B2 as a protein molecular marker for detecting liver cell carcinoma. Obvious differential expressions of the high mobility group protein B2 exist respectively in cancer cells of liver cell carcinoma and cells adjacent to the cancer cells, and thus whether a patient suffers from liver cell carcinoma can be detected based on the expression of the high mobility group protein B2 in the liver cells.

Description

technical field [0001] The invention belongs to the field of biotechnology, in particular relates to the application of high-mobility group protein B2, and more specifically relates to the application of high-mobility group protein B2 as a protein molecular marker for hepatocellular carcinoma. Background technique [0002] Hepatocellular carcinoma is the fourth most common malignant tumor in the world and the second in my country. About 250,000 patients die of hepatocellular carcinoma every year. Hepatocellular carcinoma has a high degree of malignancy, is prone to recurrence and metastasis, has a poor prognosis, and is difficult to diagnose early, which often delays the best treatment period. The incidence of liver cancer in western developed countries is low, and the basic research on liver cancer in the world is still relatively weak. However, my country is a country with a high incidence of liver cancer, and the morbidity and mortality are on the rise, and the age of ons...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/68G01N33/576G01N33/577
Inventor 曾嵘李辰徐孟杰武祎阮宏强
Owner SHANGHAI INST OF BIOLOGICAL SCI CHINESE ACAD OF SCI
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