Preparation method of chitosan

A technology of chitosan and shrimp shells, which is applied in the field of preparation of high molecular polysaccharides, can solve the problems of increasing cost and increasing the amount of acid and alkali, and achieve the effect of increasing molecular weight

Inactive Publication Date: 2011-02-16
XIAMEN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, the current technological process is difficult to obtain chitosan with high deacetylation degree and high molecular weight at the same time
In order to solve this problem, Luo Xin et al. (Luo Xin. Chitosan preparation process improvement [J]. West China Pharmaceutical Journal, 1999, 14 (1): 13-17) improved the preparation process, 2 times acid leaching Calcium salt and 2 times of deproteinization are carried out alternately, the protein and calcium salt are removed more completely, but it will also increase the amount of acid and alkali and increase the cost

Method used

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  • Preparation method of chitosan
  • Preparation method of chitosan

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] 1) Prepare raw materials: wash and dry fresh fresh shrimp shells, then pulverize them with a grinder, and pass through a sieve with a pore size of 150 μm to obtain the sieved shrimp shells;

[0023] 2) Acid treatment to remove calcium salts: Soak the sieved shrimp shells with excess hydrochloric acid under stirring conditions for 4 hours, filter, collect the shrimp shells, and wash them thoroughly with water to remove the residual hydrochloric acid in the shrimp shells to obtain the decalcified shrimp shells. Shrimp shell;

[0024] 3) Alkali treatment to remove protein: Stir and soak the decalcified shrimp shells with an excess of 10% sodium hydroxide solution at 90°C for 2 hours, cool, filter, collect the shrimp shells, and thoroughly wash them with water to remove the remaining residues in the shrimp shells. sodium hydroxide to get the shrimp shells after protein removal;

[0025] 4) Remove astaxanthin: Soak the deproteinized shrimp shells in 2% potassium permanganat...

Embodiment 2

[0034] The preparation method of chitin is the same as that of Example 1. Weigh 3 g of dried chitin and put it into a three-necked flask, react with 40% NaOH at 100° C. for 1.5 h, cool at room temperature, filter, and thoroughly wash the filtered product with water to remove residual Then put it into a three-necked flask, react with 40% NaOH at 100°C for 1.5h, cool at room temperature, filter, and wash the filtered product thoroughly with water to remove residual sodium hydroxide, then wash it twice with absolute ethanol , dried chitosan. The degree of deacetylation of the obtained chitosan is 89.19%, and the viscosity-average molecular weight is 8.91×10 5 .

Embodiment 3

[0036] The preparation method of chitin is the same as that of Example 1. Weigh 3 g of dried chitin and put it into a three-necked flask, react with 40% NaOH at 110° C. for 2 h, cool at room temperature, filter, and thoroughly wash the filtered product with water to remove residual Put sodium hydroxide into a three-necked flask, react with 40% NaOH at 110°C for 2 hours, cool at room temperature, filter, and thoroughly wash the filtered product with water to remove residual sodium hydroxide, then wash twice with absolute ethanol, and dry Get chitosan. Gained chitosan has a degree of deacetylation of 95.2%, and a viscosity-average molecular weight of 8.74×10 5 .

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Abstract

The invention discloses a preparation method of chitosan and particularly provides a preparation method of chitosan with high deacetylated degree and high molecular weight. The preparation method comprise the following steps of: firstly, preparing raw materials, removing calcium salt through acid treatment, removing protein through alkali treatment, and removing astacin to obtain chitin, and deacetylating the chitin to obtain the chitosan. Compared with the prior art, the preparation method has the advantages that the time on removing calcium and the astacin is shortened, and the molecular weight of the chitosan is improved. By using an alkali intermittent method for deacetylation by steps, the deacetylated degree of the prepared chitosan reaches 91-93 percent, and the viscometric average molecular weight of the prepared chitosan is 8.9*105-94*105.

Description

technical field [0001] The invention relates to a preparation method of high molecular polysaccharide, in particular to a preparation method of chitosan. Background technique [0002] Chitosan is an important biological material, which has attracted more and more attention due to its unique characteristics and functions. The main application areas of chitosan are medicine, food, environmental protection, agriculture, etc. At present, the demand for chitosan, especially high-purity chitosan, is increasing day by day both at home and abroad (Jiang Tingda. Chitin [M]. Beijing: Chemical Industry Press, 2003: 341-648). The method of preparing chitosan from shrimp and crab shells is mainly a chemical method, and its technological process includes acid leaching for desalination, alkali boiling for deproteinization, oxidative decolorization, and concentrated alkali for deacetylation. However, the current technological process is difficult to obtain chitosan with high deacetylation...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08B37/08
Inventor 张其清姚清清董炎明蒲曦鸣杨云
Owner XIAMEN UNIV
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