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Large yellow croaker gynogenesis induction method

A technology for gynogenesis and large yellow croaker, which is applied to the field of gynogenesis of large yellow croaker induced by sperm of yellow croaker, can solve problems such as huge and complicated workload, and achieve the effects of improving efficiency, simple genetic identification and eliminating pollution.

Inactive Publication Date: 2009-11-11
JIMEI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The disadvantage of this method is that the bred gynogenetic diploids often carry genes from the paternal species
Therefore, the disadvantage of using the same kind of sperm to induce gynogenesis

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] 1. Genetic inactivation of croaker sperm. Take the semen of yellow croaker and dilute it 30 times with the diluent, adjust the thickness of the semen to 0.5mm, and then irradiate it under the ultraviolet lamp, and the ultraviolet irradiation dose reaches 253800μW / cm 2 Stop irradiation.

[0027] 2. The start of egg gynogenesis. Take 100g of large yellow croaker eggs, add 10ml of the genetically inactivated yellow croaker semen described in step 1, mix well, add seawater at 22°C, start the gynogenesis of large yellow croaker eggs, and start timing. The control group was carried out according to the method provided by Wang Jun: take 100g of large yellow croaker eggs, add 10ml of common yellow croaker semen without genetic inactivation, mix them, add seawater at 22°C, start the development of large yellow croaker eggs, and start timing.

[0028] 3. The chromosome set doubles. When the time counted in step 2 reaches 3 minutes, transfer the developed large yellow croaker e...

Embodiment 2

[0030] 1. Genetic inactivation of croaker sperm. Take the yellow croaker semen and dilute it 35 times with the diluent, adjust the thickness of the semen to 0.6mm, and then irradiate it under the ultraviolet lamp, and the ultraviolet irradiation dose reaches 50000μW / cm 2 Stop irradiation.

[0031] 2. The start of egg gynogenesis. Take 100g of large yellow croaker eggs, add 50ml of the genetically inactivated yellow croaker semen described in step 1, mix well, add seawater at 18°C, start the gynogenesis of large yellow croaker eggs, and start timing.

[0032] 3. The chromosome set doubles. When the time counted in step 2 reaches 6 minutes, transfer the developed large yellow croaker eggs obtained in step 2 to seawater at 5°C, let them stand for 20 minutes, and then transfer them to seawater at room temperature to inflate them until they hatch. At the newly hatched larvae stage, the results of sampling by ploidy analyzer and microsatellite marker analysis showed that all samp...

Embodiment 4

[0034] Operate according to the method of Example 1, only change the starting time of 3°C seawater treatment in step 3 to 46 minutes for ovum starting. At the newly hatched larvae stage, the results of sampling by ploidy analyzer and microsatellite marker analysis showed that all samples were gynogenetic diploid and did not contain croaker gene.

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Abstract

A large yellow croaker gynogenesis induction method relates to a fish gynogenesis induction method. The induction method can exclude heterogenous genetic pollution and has simple product identification. Spotted maigre semen is diluted, thickness is adjusted, and the semen is irradiated under an ultraviolet lamp; large yellow croaker ovum and genetically inactive spotted maigre semen are mixed and then added with seawater, and large yellow croaker gynogenesis is started; and the genome of the gynogenesis haploid is doubled. Ultraviolet radiation is used for carrying out genetic inactivation on the spotted maigre semen, so as to exclude pollution of heterogenous sperm genetic material and improve the efficiency of gynogenesis. Genetic identification of the induced product is simple. Allodiploid obtained by hybridization of the large yellow croaker and the spotted maigre is unable to survive and can naturally die out during cultivation. Allotriploid obtained by hybridization doubling is extremely low in goodness of fit; and even if a small quantity of allotriploid survives, the gynogenesis diploid and the allriploid can be identified only by interspecies mark or ploidy analysis, thus simplifying genetic identification procedure of the induced product.

Description

technical field [0001] The invention relates to a method for inducing gynogenesis in fish, in particular to a method for inducing gynogenesis in large yellow croaker (Pseudosciaena crocea) with genetically inactivated sperm of croaker (Nibea albiflora). Background technique [0002] In the production of seawater fish seedlings, through artificial induction of large yellow croaker gynogenesis, the offspring will only have the female parent gene, which can quickly purify the germplasm, speed up the breeding speed, and realize parthenoculture. The invention patent with the notification number CN1586430 provides a method for inducing the diploid gynogenesis of the large yellow croaker with the sperm of the crowfish. After mixing the sperm and eggs, they are activated with sea water, and the fertilized eggs are subjected to hydrostatic shock to achieve chromosome doubling . The invention provides 6 examples, respectively adopting the sperm of two kinds of croakers, the light-col...

Claims

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Application Information

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IPC IPC(8): A01K61/00
CPCY02A40/81
Inventor 蔡明夷王志勇
Owner JIMEI UNIV
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