Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Method for preparing multipotential stem cells, reagent kit and application

A technology of pluripotent stem cells and kits, applied in the field of induced pluripotent stem cells, can solve the problem of reducing the efficiency of inducing IPS cells

Inactive Publication Date: 2009-10-07
PEKING UNIV +1
View PDF3 Cites 20 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, without using c-Myc, the efficiency of inducing IPS cells will be greatly reduced, and it cannot be repeated every time, which proves that c-Myc still plays a very critical role in reprogramming

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Method for preparing multipotential stem cells, reagent kit and application
  • Method for preparing multipotential stem cells, reagent kit and application
  • Method for preparing multipotential stem cells, reagent kit and application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

Embodiment 2

Embodiment 3

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention provides a method for efficiently inducing multipotential stem cells, six inducible factors such as Oct4 (POU5f1), Sox2, c-Myc, Klf4, UFT1, Rex1 (ZFP42), and the like and p53 gene inhibitor are induced into the cells by utilizing a slow virus carrier, thereby the method can efficiently induce and produce the multipotential stem cells.

Description

technical field The invention relates to a method, kit and application for inducing pluripotent stem cells. Background technique (1) Characteristics of stem cell pluripotency: Embryonic stem cells are called "pluripotent" stem cells because they have the ability to differentiate into three germ layer cells. In addition, embryonoma (EC) cells and embryonic germ (EG) cells have similar pluripotency to embryonic stem cells. The common characteristics of pluripotent stem cells are: large nucleoplasmic ratio, clone-like growth (human embryonic stem cells are relatively flat, while mouse embryonic stem cells are relatively raised, and the cell boundaries are not obvious), have AP enzyme activity, and specifically express SSEA4, TRA1- 60. Surface markers such as TRA1-81 (mouse embryonic stem cells also specifically express SSEA1, while human embryonic stem cells do not express SSEA1), and Oct4, Nanog, Sox2, rex1 (ZFP42), GDF3, lin28, mbd2, TEGF1 and other markers sex gene. In a...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): C12N5/06C12N5/08C12N15/11C12N15/861C12N15/867
CPCC12N2501/603C12N15/113C12N2501/40C12N5/0696C12N2501/604C12N2799/027C12N2810/6081C12N2510/00C12N2501/60C12N2501/602C12N15/111C12N2501/065
Inventor 邓宏魁赵扬尹晓磊张蔷朱芳芳刘海松杨炜烽向晨罡秦汉曲秀霞
Owner PEKING UNIV
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com