Pourable vascular tissue engineering reactor having rotating function

Abstract

The invention discloses a vascular tissue engineering reactor with rotation function. The reactor overcomes the disadvantages that the common arterial tissue engineering reactors can not simultaneously supply the vascular tissue with the culture conditions of intravascular perfusion and vascular rotation, and the mass transfer performance is poor. The vascular tissue engineering reactor comprises a liquid storage bottle, a liquid driving device, a vascular tissue culture cavity, a linear motor, a step motor, a vascular tissue transmission device, and the like, realizes the vascular tissue rotation in the course of intravascular perfusion culture, and has good mass transfer performance.

Description

technical field [0001] The invention relates to the fields of cell culture and tissue engineering, and more specifically relates to a vascular tissue engineering reactor with the function of vascular rotation. Background technique [0002] So far, autografts, allogeneic blood vessels, xenograft blood vessels, and artificially synthesized blood vessels have not been able to become ideal arterial blood vessel substitutes (especially blood vessels with a caliber of less than 6mm), but in recent years, arterial blood vessel tissue engineering bioreactors have been used to construct And research into tissue-engineered arterial vessels brings hope to the field. At present, the research content of bioreactors for arterial tissue engineering mainly includes: [0003] 1. Realize and control the uniform and high-density seeding of cells in the scaffold for vascular tissue: recent studies have found that direct perfusion bioreactors are superior to static, rotary stirring and other no...

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Problems solved by technology

[0012] In summary, there are relatively large defects in the existing vascular tissue engineering reactors: the first is that factors such as flow impedance, compliance (that is, the elasticity and buffering capacity of the vessel wall), resistance, flow inertia, etc. The simple simulation of the flow dynamics environment cannot provide a near-physiological flow environment similar to the pulsating flow state of blood in the artery; the second is that it cannot provide the vascular tissue with mechanical mechanisms such as periodic axial stretching, radial stretching, and twisting that approximate the physiological state at the same time. The third is that most of them use the method of no wall rotation, which needs to be improved in terms of uniform cell, high-density seeding and mass transfer.

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