Preparation of magnetic silicon dioxide microsphere with metallic ion chelated surface

Abstract

The invention relates to a method for preparing magnetic silicon dioxide microspheres with the surfaces chelated with metal ions; the method of the inventioncomprises the following steps of preparation of the magnetic silicon dioxide microspheres, epoxidation of the magnetic silicon dioxide microspheres, carboxylation of the magnetic silicon dioxide microspheres and chelation technique of metal ions. Compared with the existing preparing method, the method of the invention has the advantages of reasonable design, simple operation and large amount of chelation of metal, etc. The magnetic silicon dioxide microspheres with the surfaces chelated with the metal ions, which are prepared by adopting the method, contain cobalt ions, have stronger bonding force with the modified microspheres so as to lead the cobalt ions to be not easy to leak out, and have higher selectivity and proper bonding strength with histidine on the protein, thus leading the magnetic silicon dioxide microspheres with the surfaces chelated with the metal ions to have high selectivity to hexameric histidine tag protein, and being easy to realize the separation and the purification of the target protein; the magnetic silicon dioxide microspheres with the surfaces chelated with the metal ions, which are prepared by adopting the method, can be used for separating the hexameric histidine tag protein.

Description

technical field The invention belongs to the technical field of materials, and in particular relates to a material used for isolating hexahistidine protein from Escherichia coli. Background technique Biomagnetic separation technology is a high-efficiency separation technology developed on the basis of traditional magnetic separation technology, which is applied to organisms such as cells, bacteria, nucleic acids and proteins. It uses the characteristics of magnetic or magnetically labeled organisms to perform qualitative movements under the action of an external magnetic field to extract, enrich, separate and purify target organisms. Early work mainly focused on the development of preparation techniques for magnetic markers, including the adsorption of magnetic nanoparticles to polymer microspheres such as agarose, polyacrylamide, polyglutaraldehyde, and polyvinylaldehyde by immersion in ferrofluid. The microspheres are characterized by a uniform particle size distribution ...

AI Technical Summary

Problems solved by technology

In common complexing agents, IDA is three-coordinated, and the force between it and metal ions is relatively weak, which can easily cause the problem of missing metal ions. N, N, N'-tris(carboxymethyl)ethylenediamine TED It is a five-coordinate, and has a strong interaction with metal ions. Since only one bond interacts with protein, the interaction with protein is too weak.

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