Aculeus type tissue slice glassivation refrigeration carrier and refrigeration method for ovary tissue

Abstract

An acupuncture-type tissue slice vitrification freezing carrier is composed of a freezing tube with one end being closed and the other end being open, and an acupuncture device. The acupuncture device comprises a needle head and a needle disposed on the needle head and used for threading tissue slices. A section matching with the internal shape and size of the open end of the freezing tube is disposed on the needle head. After the acupuncture device and the freezing tube are combined, the needle is positioned in the freezing tube, and the needle head is adopted as a tube plug of the freezing tube to seal the open end of the freezing tube. A plurality of tissue slices (such as ovary tissue slices and liver tissue slices) can be threaded in a row on the needle at intervals by the carrier soas to be subjected to each operation of the vitrification freezing and storage, and a plurality of sample tissues can be subjected to the vitrification freezing and storage simultaneously, with simple operation and high efficiency, so that the sample tissues can be dehydrated and refrigerated under the same conditions. The storage of the ovary multicellular ingredients (such as the storage of interstitial cells and extracellular matrix ingredients) is superior to the programmed slow freezing and the carrier-free vitrification freezing method.

Description

technical field [0001] The invention belongs to the field of vitrification cryopreservation, in particular to a vitrification freezer and freezing method for organs or tissues such as ovaries and livers. Background technique [0002] With the advancement of cryopreservation technology, freezing technology is not limited to freezing cells, gametes or embryos. The cryopreservation of tissues and organs is the development trend of cryogenic technology. Vitrification is an ultra-rapid freezing method proposed in recent years. It takes less time than programmed freezing, is convenient and cheap, and has no ice crystal damage. Studies believe that it is more suitable for cryopreservation of tissues or organs with different cell components, but vitrification has disadvantages. Yes: The concentration of cryoprotectant required is high, resulting in increased cytotoxicity, which limits its application in the preservation of human tissues and organs. Improving the freezing carrier o...

AI Technical Summary

Problems solved by technology

Vitrification is an ultra-rapid freezing method proposed in recent years. It takes less time than programmed freezing, is convenient and cheap, and has no ice crystal damage. Studies believe that it is more suitable for cryopreservation of tissues or organs with different cell components, but vitrification has disadvantages. Yes: The concentration of cryoprotectant required is high, resulting in increased cytotoxicity, which limits its application in the preservation of human tissues and organs

These methods either cannot fully expose the tissue to liquid nitrogen, or the cryoprotectant residue on the tissue surface will reduce the freezing rate, especially when multiple samples are frozen at the same time, it will increase the difficulty of operation, and it is difficult to ensure that each sample is dehydrated and frozen under the same conditions

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