Particle analyzer of sheath-flow impedance method

Abstract

The invention puts forward a sheath flow impedance particle analyzer, comprising a counting cell, a counting circuit, a latter sheath partition cell and a liquid waste partition cell; wherein, the counting cell comprises a former cell and a latter cell; the former cell comprises a particle suspension liquid inlet and a former sheath liquid inlet; the latter cell comprises a latter sheath liquid inlet and a liquid waste outlet; a hole is arranged between the former cell and the latter cell which are connected with the counting circuit through electrode; the latter sheath liquid stored in the latter sheath partition cell can automatically and continuously flow into the latter cell under the gravitation and the internal negative pressure in the latter cell; the latter sheath partition cell can make the internal liquid passageway, which connects the inlet and the outlet of the latter sheath partition cell, insulated by the air; the liquid waste partition cell can make the internal liquid passageway insulated, which connects the inlet and the outlet of the liquid waste partition cell. The invention has the advantages of preventing the reflowing of the sample liquid in the process of detecting, reducing the signal noise effectively, increasing the detecting sensitivity of the particle and avoiding the electromagnet noise introduced to the counting cell from the latter sheath partition cell or the liquid waste partition cell.

Description

technical field [0001] The invention relates to a sheath flow impedance method particle analyzer, which can use the sheath flow impedance method to detect and analyze the number, volume, etc. of particles and other applications. Background technique [0002] Particle count and volume detection using sheath flow impedance methods can be traced back to 1974 US Patents US 3,793,587 and US 3,810,010. Its common basic components are: front pool, back pool, small hole, sample needle, electrode, detection circuit; the front pool and back pool are connected by a small hole, the sample needle is coaxial with the small hole, and its outlet is placed in the front pool The position is very close to the small hole, so that the detected particle suspension (called the sample flow) can be input into the counting cell. The front cell and the back cell each have a particle-free conductive liquid source, which is called the front sheath flow. and the rear sheath flow; the pressure of the fro...

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Problems solved by technology

[0005] U.S. Patent No. 5,905,214 is driven by the method of "positive pressure drive". The power to push the rear sheath fluid into the rear pool comes from the positive pressure in the upstream pool. To ensure that the positive pressure can be transmitted to the rear pool, it is necessary to ensure that the fluid along the way The sealing of the road and the continuous supply of liquid, so it is difficult to ensure that no external interference signals are introduced into the rear pool

U.S. Patent US 6,909,269 uses the method of placing the counting cell in a special shielding box, which has a certain shielding effect, but it cannot prevent the backflow of the sample after entering the back cell and generate false signals

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