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Method for extracting residual DNA in edible vegetable oil

A technology of edible vegetable oil and extraction method, which is applied in the field of extraction of residual DNA in edible vegetable oil, and can solve problems such as insufficient removal ability, unsatisfactory extraction effect, and need to import

Inactive Publication Date: 2011-03-30
上海新云数据技术有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the extraction effect of plant DNA rich in phenols and fats is not satisfactory, and the ability to remove impurities such as pigments and choline is not enough, so that the final DNA solution is light yellow and the amplification effect is poor.
[0005] The High Pure GMO Sample Preparation Kit, a special kit for food DNA extraction produced by ROCHE in Switzerland, can be used for the extraction of residual DNA in edible oils and fats, but it is expensive and needs to be imported, so it is not suitable for commercial market monitoring

Method used

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  • Method for extracting residual DNA in edible vegetable oil
  • Method for extracting residual DNA in edible vegetable oil

Examples

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Embodiment Construction

[0023] Now take the soybean salad oil, corn salad oil, rapeseed blend oil and cottonseed oil purchased in the supermarket as an example, and the non-limiting examples are described as follows:

[0024] (1) Instrument

[0025] MINI small high-speed centrifuge Germany Eppendorf company;

[0026] 3K15 high-speed refrigerated centrifuge from Sigma, Germany;

[0027] Mycycler TM Thermal cycler type PCR amplification instrument American Bio-Rad company;

[0028] Power Pac Basic type electrophoresis instrument American Bio-Rad company;

[0029] Gel Doc TM XR gel imaging system American Bio-Rad company;

[0030] MLS-3750 autoclave Japan SANYO company;

[0031] Ultrospec3100pro type ultraviolet spectrophotometer Sweden Pharmacia biotech company;

[0032] ELGA PURELAB Classic DI ultrapure water meter, British ELGA Lab Water Company;

[0033] Pipette gun 5mL, 1mL, 200μL, 100μL, 20μL, 10μL, 1μL Eppendorf Germany;

[0034] Ultra-clean workbench, ultra-low temperature refrigerator,...

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PUM

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Abstract

A method for extracting residual DNA in edible vegetable oil is carried out by transferring DNA in oil and fat and enriching into aseptic water by water-emulsifying method, adding extracted buffer liquor with CTAB, Tris, NaCl and EDTA into water bath between 63-67 deg. C, keeping temperature for 30-60 mins to obtain aqueous extract, adding purifying agent with phenol, trichloromethane and isopropyl alcohol into aqueous extract, purifying, removing organic phase and aqueous phase to obtain purified liquor, adding precipitant into pre-cooled purified liquor, agitating, laying aside at -25--18 deg. C, precipitating while eluting DNA, separating, washing, and drying to obtain final product. It can be used to extract DNA from soya bean oil, corn oil, rapeseed oil and cottonseed coil. It has high-purity template DNA and need to purify.

Description

1. Technical field [0001] The invention relates to a unit process in a chemical process, in particular to a physical separation process, specifically a method for extracting residual DNA in edible vegetable oil. 2. Background technology [0002] At present, genetically modified technology has been promoted and applied in agriculture, such as genetically modified cotton (insect-resistant cotton), genetically modified soybeans, etc. Due to the safety problems of genetically modified plants, the labeling of genetically modified crops has become a major focus of public attention. More than 30 countries have formulated corresponding laws and regulations to label genetically modified crops and their derivative products. With the further opening of my country's market, the possibility of some unlabeled and certified genetically modified organisms and their deep-processed products entering the domestic market is greatly increased, which urgently requires appropriate testing methods ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07H21/04C07H1/08
Inventor 陈彦王亚
Owner 上海新云数据技术有限公司
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