Method for producing alcohol and feed by utilizing seaweed chemical waste material
A seaweed and chemical technology, applied in the field of marine resources and biology, can solve problems such as environmental pollution, lack of treatment methods and utilization methods, residues and wastes, and achieve the effect of solving environmental pollution problems
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Embodiment 1
[0030] Embodiment 1: Kelp waste residue, serial fermentation.
[0031] The method for producing ethanol and feed by utilizing seaweed chemical waste, consists of the following steps:
[0032] (1) Seaweed chemical waste selection: choose kelp waste residue, according to the water content of the waste residue, add an appropriate amount of water to make a suspension with a residue concentration of 30% in weight percentage, adjust the pH to 5 to 6 with dilute acid solution, and set aside .
[0033](2) Strain selection: select cellulase producing bacteria: Trichoderma viride (Trichoderma viride) AF93252 provided by the China Typical Culture Collection Center of Wuhan University, the preservation number is CCTCC NO.AF93252; Saccharomyces cerevisiae strain: Angelica, from Supermarket purchases.
[0034] (3) Preparation of liquid fermentation strains: use the cellulase-producing bacterial strain described in step (2), under aseptic conditions, through test tube slopes, shake flasks,...
Embodiment 2
[0042] Embodiment 2: Sargassum waste residue, serial fermentation
[0043] The method for producing ethanol and feed by utilizing seaweed chemical waste, consists of the following steps:
[0044] (1) Seaweed chemical waste selection: select Sargassum waste residue, add appropriate amount of water according to the water content of the waste residue to make a suspension with a residue concentration of 25% by weight, adjust the pH to 5.5 with dilute acid solution, and set aside.
[0045] (2) Strain selection: Trichoderma viride AF93252, provided by China Center for Type Culture Collection (CCTCC) of Wuhan University, [Accession No.] AF93252; Saccharomyces cerevisiae strain: Angelica, purchased from a supermarket.
[0046] (3) Preparation of liquid fermentation strains: use the cellulase-producing bacterial strain described in step (2), under aseptic conditions, through test tube slopes, shake flasks, first-level seed tanks, and second-level seed tanks to gradually amplify and cul...
Embodiment 3
[0054] Embodiment 3: kelp raw material, serial fermentation
[0055] The method for producing ethanol and feed by utilizing seaweed chemical waste, consists of the following steps:
[0056] (1) Selection of seaweed chemical waste: choose dry kelp to pulverize, add appropriate amount of water to make a suspension with a slag concentration of 35% by weight in a ratio of about 1:40, adjust the pH to 5 with dilute acid solution, and set aside .
[0057] (2) Strain selection: Trichoderma viride (Trichoderma viride) AF93252 provided by the China Center for Type Culture Collection (CCTCC) of Wuhan University, [preservation number] AF93252; Saccharomyces cerevisiae strain: Angelica, purchased from a supermarket.
[0058] (3) Preparation of liquid fermentation strains: use the cellulase-producing bacterial strain described in step (2), under aseptic conditions, through test tube slopes, shake flasks, first-level seed tanks, and second-level seed tanks to gradually amplify and cultivat...
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