Method for separating and purifying oligosaccharide in high purity

A technology for separation and purification of oligosaccharides, applied in oligosaccharides, chemical instruments and methods, sugar derivatives, etc., can solve problems such as difficult sugar separation and purification, high cost, and non-recyclable activated carbon

Inactive Publication Date: 2008-12-03
NORTH CHINA PHARMA GROUP CORP
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Over the years, except for molecular sieves and activated carbon, it is difficult to separate and purify sugars with conventional resins. See Chapter 9, Section 6 of "Natural Product Chemistry": Extraction and separation of sugars, pages 469-475, Chinese invention patent application number 95103433.2 , Invention name: Production process for extracting soybean oligosaccharides by membrane separation method, China Invention Patent Application No. 98125920.0, Invention name: Oligosaccharide purification method, due to the high cost of molecular sieve as a separation medium, activated carbon has unrecyclable and other unfavorable factors, The application of these two separation media is limited in large-scale production
Good separation of sugars with Sephadex G-10 resin, but at a high cost

Method used

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  • Method for separating and purifying oligosaccharide in high purity
  • Method for separating and purifying oligosaccharide in high purity
  • Method for separating and purifying oligosaccharide in high purity

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0045] Conventional resin separation and purification soybean oligosaccharides of embodiment 1 special treatment

[0046] Resin pretreatment:

[0047] After the HZ001 resin is fully swollen with no salt water, it is soaked with 1-2mol / L HCl for 2 hours, and washed with water until neutral;

[0048] After soaking in 1-2mol / L NaOH for 2 hours, wash with water until neutral;

[0049] Use 1~2mol / LCaCl 2 After soaking for 2 hours, wash with water until neutral.

[0050] The glass column used for the separation medium: the ratio of diameter to height is 1:30,

[0051] The loading volume is 120ml, and the column is packed by wet method.

[0052] Resin regeneration:

[0053] After soaking with 1-2mol / L HCl for 2 hours, wash with water until neutral;

[0054] After soaking in 1-2mol / L NaOH for 2 hours, wash with water until neutral;

[0055] Use 1~2mol / LCaCl 2 After soaking for 2 hours, wash with water until neutral and set aside.

[0056] Commercially available soybean oligos...

Embodiment 2

[0057] Conventional resin separation and purification soybean oligosaccharides of embodiment 2 special treatment

[0058] Same as Example 1, except that 0.5~1mol / L sulfuric acid is used to replace 1~2mol / LHCl, and 5~8% ammonia water is used to replace 1~2mol / LNaOH to obtain a finished product of oligosaccharides with a stachyose content of 89%, with a primary recovery rate of 65%. %. The finished product atlas is the same as attached drawing 2.

Embodiment 3

[0059] Example 3 Sephadex G-10 separation and purification of soybean oligosaccharides

[0060] Sephadex G-10 Resin Resin Pretreatment:

[0061] After the resin is fully swollen with water, the column is wet-packed.

[0062] Glass column used for Sephadex G-10 resin: diameter to height ratio is 1:25

[0063] Commercially available soybean oligosaccharide syrup (stachyose content is 30%), see accompanying drawing 1, after diluting 5 times (stachyose unit: 24800ug / ml) take 1ml sample, flow rate is 1 / 10-1 / 15BV / hr, ELSD follow-up detection, collect the effective parts and concentrate under reduced pressure to obtain a finished oligosaccharide with a stachyose content of 90% and a recovery rate of 65%, which is suitable for diabetics and obese patients. See attached drawing 3 for the finished product map.

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Abstract

A process for separating and purifying the high-purity oligose includes treating the resin HZ001 sequentially by acid, alkali and salt solution, and using the treated resin to prepare high-purity oligose from low-purity one. The process for treating and regenerating resin HZ001 is also disclosed.

Description

Technical field: [0001] The invention belongs to the field of separation and purification of sugars, more precisely, the separation and purification of oligosaccharides. Background technique: [0002] High-purity soybean oligosaccharides and fructooligosaccharides are indigestible sugars. After human ingestion, they can be directly used by beneficial bacteria such as bifidobacteria in the large intestine. Increase, acidify the intestinal tract, thereby inhibiting the production of spoilage bacteria, inhibiting the rise of cholesterol, comprehensively improving the intestinal environment, and improving the absorption of trace elements such as calcium and magnesium, see "Chinese Journal of Biochemical Medicine" 1997 No. 18 Volume No. 5, pp. 268-271, "Research on the Physiological Activity of Oligosaccharides"; Efficacy of hyperlipidemia". The purity of soybean oligosaccharide products is as high as 80% (stachyose + raffinose), and the fructooligosaccharides (kestose + kestos...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07H3/06C07H1/06
Inventor 刘刚叁张雪霞林旸赵辉
Owner NORTH CHINA PHARMA GROUP CORP
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