Use of icariside II in manufacture of products for preventing or treating male or female sexual dysfunction
a technology of icariside and product, which is applied in the direction of biocide, plant/algae/fungi/lichens, plant compositions, etc., can solve the problems of pde5i showing no therapeutic effect on the pathological changes of the erectile tissue of the penis, the inability to treat the pathological changes of the penis, and the inability to induce the erectile tissue to develop, so as to improve the erectile tissue of the patien
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example 1
Preparation of Icariside II Extracts and Icariside II
Extraction Method (1)
Extracts Comprising Icariside II
[0035]Five hundred gram dried aerial part of Epimedium were ground and extracted with 10 L of 70% EtOH at 80 C.° for one hour, three times, three back, under reflux. Then the extract was filtered and the filtrate was concentrated. The ethanol extract was then suspended in 4 times water and allowed to sit for ahs to remove the chlorophyll and other waxes. Then after extraction with n-butanol for times, the extracts were combined and concentrated. Then the extracts were subjected to 40-60 mesh polyamide column and eluted with 30% ethanol. The elutes were concentrated and resolved in 70% ethanol (1:20) and kept still for 8 hours. After filtration, icariin was obtained. Then icariin was recrystallized with anhydrous ethanol (1:15) and extract comprising icariside II was obtained. HPLC analysis conformed that the content of icariside II is 50.2%.
Extraction Method (2)
Separation and Pu...
example 2
The Effect of Icariside II on Penile Nerve Regeneration in Old Rats
Materials and Methods
[0039]The 24-month-old Sprague-Dawley male rats were used. All experimental animals used in this study were under a protocol approved by the Institutional Animal Care and Use Committee. Rats were anesthetized by intraperitoneal injection of pentobarbital sodium (200 mg / kg). After midline incision on abdomen, major pelvic ganglion was carefully dissected from prostate. MPG culture and treatment: the Bilaterally MPG was rapidly removed and after washing with PBS, DCR were stripped from MPG and cut into 3 pieces with the same size.
[0040]Reduced Growth Factor Matrigel (RGFM) was diluted three times with serum free RPMI-1640 medium and placed in the 35 mm Petri dish on ice. The diluted RGFM were poured onto the cold sterilized glass cover slip and incubated at 37° C. for 1 hour in 35 mm Petri dishes to make the RGFM steady. Then each MPG was embedded in 40 μl of pre-cooled RGFM and maintained at 37° C...
example 3
Effect of Icariside II on NOS Enzyme Activity in Vascular Endothelial Cells (PAE)
Method and Material
[0045]Porcine vascular endothelial cells (PAE) were provided by the Institute of Urology, Peking University. The determination of the effect of icariside II on NOS activity in the endothelial cell is conducted using NOS kit (Cayman Chemical company, Ann Arbor, Mich., USA). Vascular endothelial cells (PAE) were treated with different concentrations of icariside II (0, 10−9, 10−8, 10−7, 10−6, 10−5 and 10−4 M) (sildenafil as the control group). 180 μl lysate was added into each culture well. 20 μl supernatant was used for protein concentration assay with BCA protein kit. And, 100 μl supernatant was analyzed for NOS activity with NOS kit (Cayman Chemical company, Ann Arbor, Mich., USA). NOS activity (units / mg)=[(valueNos-measure−valueNOS-blank)÷(38.3×106)]×[(2.15+supernatant volume)÷(15×supernatant volume)]÷[protein concentration of each Petri dish].
Result
[0046]The activity of NOS enzyme ...
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