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Purified plyporus laccase and nucleic acid for coating the laccase

A laccase and coding technology, applied in the fields of nucleic acid fragments of laccase, purified enzymes, and phenol oxidase, and can solve problems such as low yields

Inactive Publication Date: 2007-08-01
NOVO NORDISK AS
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Previous attempts to express basidiomycete laccases in recombinant hosts resulted in extremely low yields

Method used

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  • Purified plyporus laccase and nucleic acid for coating the laccase
  • Purified plyporus laccase and nucleic acid for coating the laccase
  • Purified plyporus laccase and nucleic acid for coating the laccase

Examples

Experimental program
Comparison scheme
Effect test

Embodiment

[0053] I. Isolation of P0lyporus pinsitus laccase

[0054] Materials and methods

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PUM

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Abstract

The present invention relates to isolated nucleic acid constructs containing a sequence encoding a Polyporus laccase, and the laccase proteins encoded thereby.

Description

[0001] This application is a divisional application of the application No. 95194301.4 submitted on June 15, 1995, and the invention title is "purified Polyporus laccase and nucleic acid encoding the enzyme". technical field [0002] The present invention relates to isolated nucleic acid fragments encoding a fungal oxidoreductase, and purified enzymes derived from the fragments. More particularly, the present invention relates to nucleic acid fragments encoding a phenoloxidase, in particular a laccase from Basidiomycetes-Polypores. Background technique [0003] Laccases (benzenediol:oxygen oxidoreductases) are multi-copper enzymes that catalyze the oxidation of phenolics. Oxidation of suitable phenolic substrates by laccases leads to the production of aryloxy intermediates that undergo terminal polymerization to form a mixture of dimerization, oligomerization, and polymerization reaction products. This reaction actually plays an important role in the biosynthetic pathways le...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N15/53C12N9/02C12R1/645C12N15/70C12N15/80C12N1/15C12N15/09A61K8/00A61K8/66A61Q5/10C09B53/02C12N15/00C12R1/66D06M16/00D06P1/00D06P1/32D21C5/00
CPCC12N9/0061D06M16/003D06P1/32A61K8/66D21C5/005D06P1/0048D06P1/0004C12N15/80A61Q5/10
Inventor D·S·亚夫尔F·许H·达尔伯吉P·施耐德D·A·阿斯林格
Owner NOVO NORDISK AS
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