Liquid nitrogen automatic filling and data acquisition linkage device and method for cryoelectron microscope

Abstract

The invention relates to a liquid nitrogen automatic filling and data acquisition linkage device and method for a cryoelectron microscope. The liquid nitrogen automatic filling and data acquisition linkage device comprises a liquid nitrogen supply unit, a liquid nitrogen conveying unit and a data acquisition linkage control unit; the liquid nitrogen conveying unit is fixedly arranged at the position of a lens cone of the cryoelectron microscope and used for conveying liquid nitrogen output by the liquid nitrogen supply unit into a liquid nitrogen tank at the rear end of a sample freezing and conveying rod, a sample to be detected is loaded at the front end of the sample freezing and conveying rod, and the sample freezing and conveying rod is inserted into an electron microscope of the cryoelectron microscope; and the data acquisition linkage control unit is used for controlling the liquid nitrogen supply unit to automatically fill the liquid nitrogen into the liquid nitrogen tank and controlling the data acquisition process of the data acquisition software of the cryoelectron microscope in a linkage manner. The device and method can be widely applied to the field of freezing electron microscopes.

Description

technical field [0001] The invention relates to the field of sample detection, in particular to the field of cryo-electron microscopes, in particular to an automatic liquid nitrogen filling and data acquisition linkage device and method for cryo-electron microscopes. Background technique [0002] In recent years, after observing the microstructure of biological samples such as cells, proteins, and chemical material samples such as emulsions and vesicles in situ using a cryo-electron microscope (usually at a temperature of 90K), the single particle analysis (SPA) technique has been applied. , cryo-electron tomography (cryo-electron tomography, cryo-ET), etc. can be analyzed to obtain high-resolution three-dimensional structures, and has become a very important technology in biological and chemical research. When the cryo-electron microscope collects the microstructure of the sample at low temperature, a frozen sample stage cooled by liquid nitrogen is required, and the data c...

AI Technical Summary

Problems solved by technology

[0006] In order to solve the problem that the above-mentioned sample rod cannot automatically collect data for a long time, the present invention provides a liquid nitrogen automatic filling and data collection linkage device and method for cryo-electron microscopy, which can provide a stable low-temperature environment for the frozen sample stage, and is compatible with Data acquisition linkage, able to automatically collect data for a long time

Images