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Application of tempol in the preparation of drugs for the treatment of polycystic ovary syndrome

A technology for polycystic ovary syndrome, applied in the field of application in the preparation of drugs for the treatment of polycystic ovary syndrome, to achieve broad application prospects, improve PCOS, alleviate the effect of abundance and

Active Publication Date: 2021-12-14
BEIJING OBSTETRICS & GYNECOLOGY HOSPITAL CAPITAL MEDICAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Tempol (4-hydroxy-2,2,6,6-tetramethylpiperidine-1 oxygen radical) is an azacyclohexane nitroxide with strong stability, high cell membrane permeability and non-toxicity At present, there is no report about the application of Tempol in the preparation of drugs for the treatment of polycystic ovary syndrome

Method used

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  • Application of tempol in the preparation of drugs for the treatment of polycystic ovary syndrome
  • Application of tempol in the preparation of drugs for the treatment of polycystic ovary syndrome
  • Application of tempol in the preparation of drugs for the treatment of polycystic ovary syndrome

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0074] The construction of embodiment 1 animal model

[0075] Three-week-old SD rats were divided into three groups: control group (Oil+PBS group), PCOS model group (DHEA+PBS group), Tempol treatment group (DHEA+Tempol group). There were 6-11 rats in each group, and the construction method of the rat model in the control group was as follows: 200 μL of sesame oil was injected subcutaneously, and after 21 days of continuous injection, PBS was injected intraperitoneally for 12 consecutive days; Hydroepiandrosterone (DHEA) (Solarbio, D8950) 6mg / 100g, 200 μ L, after continuous injection for 21 days, intraperitoneal injection of PBS for 12 consecutive days; the construction method of the Tempol treatment group rat model is as follows: subcutaneous injection of DHEA ) 6mg / 100g, 200μL, after continuous injection for 21 days, Tempol (30mg / kg) (Selleck Chemicals LLC#S2910, Houston, TX, USA) was injected intraperitoneally for 12 consecutive days. Weighing was performed weekly during th...

Embodiment 2

[0077] Example 2 The observation of rat estrous cycle, the observation of ovarian histomorphology and the measurement and analysis of serum hormones 1, the observation of rat estrous cycle

[0078] Rats are animals with multiple estrus throughout the year and have a relatively stable sexual cycle, which is divided into: preestrus (P), estrus (E), postestrus (M) and diestrus (D). The vaginal mucosa of each period is Typical changes occur, which stage can be judged according to the cytological characteristics of the vaginal smear. In this embodiment, 3 rats in the control group (Oil+PBS group), PCOS model group (DHEA+PBS group), and Tempol treatment group (DHEA+Tempol group) three groups of experimental animals were taken from the vagina at the same time every afternoon. The cell smears were stained with Wright's staining solution (LEAGENE, 1029A20) for 10 consecutive days to observe the cell morphology and record and count the experimental results respectively.

[0079] 2. HE ...

Embodiment 3

[0086] Example 3 Statistical analysis on biomarker level

[0087] 1. 16S rRNA gene sequencing

[0088] Total bacterial DNA was extracted from feces using a DNA isolation kit (MO BIO Laboratories, Carlsbad, CA, USA). The quality and quantity of DNA were evaluated by the ratio of 260nm / 280nm and 260nm / 230nm. DNA was then stored at -80°C until further processing. The V3-V4 region of the bacterial 16S rRNA gene was amplified with common primers (SEQ ID NO.1-SEQ ID NO.2) in combination with the adapter sequence and the barcode sequence. Thermal cycling conditions were: initial denaturation at 95°C for 5 min, followed by 15 cycles of denaturation at 95°C for 1 min, 50°C for 1 min and 72°C for 1 min, and finally extension at 72°C for 7 min. The first step PCR product is purified by VAHTSTM DNA cleaning beads. The second round of PCR was performed in a 40 μL reaction consisting of 20 μL 2× Phμsion HF-MM, 8 μL ddH 2 O. 10 μM of each primer and 10 μL of the PCR product from the fir...

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Abstract

The invention discloses the application of Tempol in the preparation of drugs for the treatment of polycystic ovary syndrome. Through animal experiments, it is first found that Tempol can reduce the level of androgen in mammals, restore the ovulation function of mammals, improve polycystic changes in ovaries, and relieve intestinal flora. Changes and alleviate the differential changes of serum metabolites, and then can significantly improve PCOS, and has the advantages of significant curative effect, the proposal of the present invention provides a new technical means for the treatment of PCOS, clinical treatment of PCOS and / or Or adjuvant therapy has very important significance and broad application prospects.

Description

technical field [0001] The invention belongs to the field of biomedicine, and in particular relates to the application of Tempol in the preparation of medicines for treating polycystic ovary syndrome. Background technique [0002] Polycystic ovary syndrome (PCOS) is an endocrine disorder and metabolic abnormality with complex etiology and various clinical manifestations. Hormone production excess), the main clinical manifestations are irregular menstrual cycle, infertility, hirsutism and / or acne, which is widely considered to be a combination of ovulatory dysfunction, excess androgen levels and ovarian polycystic changes, excluding the possibility of causing similar Depression, lack of self-confidence and other mood disorders often occur. Its etiology and specific pathogenesis have not yet been elucidated, and studies suggest that it may be related to certain genetic variations, environmental factors, and metabolic abnormalities. Currently, about 4% to 8% of women worldwid...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K31/445A61K35/74A61K49/00A61P15/00A61P15/08A61D7/00
CPCA61K31/445A61K35/74A61K49/0008A61P15/00A61P15/08A61D7/00A61K2300/00
Inventor 李天鹤阴赪宏刘瑞霞张婷婷
Owner BEIJING OBSTETRICS & GYNECOLOGY HOSPITAL CAPITAL MEDICAL UNIV
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