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Application of muscle stem cells in the preparation of anti-inflammatory drugs

An anti-inflammatory drug and stem cell technology, applied in the direction of anti-inflammatory agents, drug combinations, antipyretics, etc., can solve problems that have not been clearly concluded, and achieve the effect of reducing weight loss

Active Publication Date: 2021-07-27
SUZHOU UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, whether muscle stem cells, as the core cell components of skeletal muscle, are the cell source of these immunomodulatory molecules is still unclear in the scientific community

Method used

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  • Application of muscle stem cells in the preparation of anti-inflammatory drugs
  • Application of muscle stem cells in the preparation of anti-inflammatory drugs
  • Application of muscle stem cells in the preparation of anti-inflammatory drugs

Examples

Experimental program
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Effect test

Embodiment 1

[0172] Example 1: MuSC alleviates DSS-induced colitis

[0173] To explore whether MuSCs have anti-inflammatory therapeutic effects, we injected expanded MuSCs intravenously into mice with dextran sodium sulfate (DSS)-induced colitis. The results showed that a single intravenous injection of MuSC significantly improved the disease symptoms of colitis compared with PBS-treated control colitis mice. The weight loss of the mice slowed down, and the disease activity index decreased ( figure 2 A and 2B). In addition, the reduced degree of atrophy of colon tissue in mice also indicated the beneficial therapeutic effect of MuSC ( figure 2 C). At the same time, through histopathological examination, it was found that the intestinal wall thickening, crypt damage, and colonic inflammatory cell infiltration in mice intravenously infused with MuSC were alleviated to varying degrees ( figure 2 D). Serum IL-6 concentration, an important indicator for monitoring the disease process of...

Embodiment 2

[0175] Example 2: MuSC confers anti-inflammatory phenotypic characteristics on macrophages

[0176] As the main pathogenic cell population of colitis, inflammatory macrophages have become potential targets for the development of new therapeutic drugs for colitis. To this end, we used MuSC supernatants to treat macrophages and detect changes in macrophage phenotypic characteristics. The results showed that anti-inflammatory genes (Arg-1, Chil3, CD206 and TGF-β) were significantly increased in macrophages treated with MuSC supernatant ( image 3 A). Even under the stimulation of inflammatory factors (lipopolysaccharide and interferon-γ), macrophages treated with MuSC supernatant did not show high levels of expression of inflammatory pathogenic factors (IL-1β and IL-6) ( image 3 B and 3C). Relevant reports at home and abroad have pointed out that the increased expression of macrophage PD-L1 is crucial for the outcome of disease inflammation and the restoration of immune homeo...

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Abstract

The invention discloses an application of muscle stem cells in the preparation of anti-inflammatory drugs. The muscle stem cells of the present invention or their supernatants are mediated by anti-inflammatory macrophages, and their anti-inflammatory properties are shaped during the maturation of macrophages, which can effectively treat inflammatory diseases in which inflammatory macrophages are the main pathogenic cell population . For example, treating colitis mice with muscle stem cells or their supernatants in the present invention can effectively reduce the disease symptoms of weight loss, diarrhea, hematochezia, intestinal tissue thickening and edema, structural damage and immune cell infiltration.

Description

technical field [0001] The invention relates to the application of muscle stem cells in the preparation of anti-inflammatory drugs, belonging to the technical field of immune regulation. Background technique [0002] Skeletal muscle is the largest tissue and organ in the human body, accounting for about 35% of the body weight. In addition to providing the necessary energy for individual sports, it also participates in the body's glucose and lipid metabolism and the precise regulation of body temperature. In addition to myogenic cells, immune cells are abundant in healthy skeletal muscle tissue. adult body per mm 3 Muscle tissue contains 500-2000 white blood cells, which is equivalent to about 10 white blood cells per liter of muscle tissue. 9 white blood cells. Although there are many types of white blood cells in muscle, the vast majority of immune cell populations are monocytes and macrophages. They surround the muscle stem cells (Muscle stem cell, MuSC) of skeletal m...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K35/34A61P29/00A61P1/00
CPCA61K35/34A61P1/00A61P29/00
Inventor 时玉舫房建凯陈永井张胜超邵常顺
Owner SUZHOU UNIV
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