A kind of optical excitation and detection method of nucleic acid amplification instrument

Abstract

The invention provides an optical excitation and detection method of a nucleic acid amplification instrument, which includes injecting nucleic acid into the first reaction chamber; the heating device works so that the nucleic acid in the first reaction chamber is thermally split, the first control valve is opened, The split nucleic acid enters the second reaction chamber through the diversion pipeline; the output control valve is opened so that the intermediate in the output storage bin flows into the second reaction chamber; the controller controls the cooling device to start working for the second reaction chamber; The temperature of the reaction chamber is cooled; the heating device in the second reaction chamber starts to work, the controller controls the opening of the second control valve, and at the same time, the controller also controls the operation of the pump body to transport the nucleic acid in the second reaction chamber back to the first reaction chamber ; The controller analyzes the data collected by the fluorescence detection unit, and calculates the relationship between the system stability value Δs and the measurement interval K to select the corresponding working mode; repeat the above reaction 20-30 times.

Description

technical field [0001] The present invention generally relates to a nucleic acid amplification instrument, and more particularly relates to an optical excitation and detection method for a nucleic acid amplification instrument. Background technique [0002] Nucleic acid amplification is a general term for a large class of technical methods, currently including conventional PCR, real-time fluorescent PCR, isothermal nucleic acid amplification technology, etc. Nucleic acid amplification is very useful in molecular biology and is widely used in biology, therapeutics, diagnostics, forensic And every aspect of the study has broad applicability. Typically, one or more primers are used to generate an amplicon from a starting template, wherein the amplicon corresponds to or is complementary to the template from which the amplicon was generated. Multiplex amplification also simplifies the process and reduces costs. [0003] The existing nucleic acid amplification technology mainly ...

AI Technical Summary

Problems solved by technology

[0004] The existing nucleic acid amplification instrument is not highly intelligent. In the process of nucleic acid amplification, the addition of primers, buffers, dNTP and other components requires manual addition by staff; at the same time, in the process of nucleic acid amplification, it is often necessary To amplify nucleic acid multiple times, temperature control is required in each nucleic acid amplification process, and these operations need to be performed manually, which is time-consuming and laborious, and is not conducive to production and research; therefore, the present invention provides a nucleic acid amplification method. Intensified optical excitation and detection methods to at least partially address the above-mentioned problems

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