A kind of rooting culture and seedling raising method of Thorn elm tissue culture seedling
A technology of rooting culture and rooting medium, which is applied in the field of rooting culture and seedling cultivation of Ulmus chinensis tissue culture seedlings, which can solve the problems of slow seedling growth, few elm thorns, and low reproduction coefficient, and achieve the improvement of cultivation survival rate and the degree of lignification Improve the effect of dark green leaves of plants
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Embodiment 1
[0026] A method for rooting culture and seedling raising of Ulmus chinensis tissue culture seedlings is characterized in that it comprises the following steps:
[0027] 1) Selection of rooting materials: select the subcultured tissue-cultured seedlings of Ulmus chinensis with a plant height of 3 cm and a subculture period of 30 days, cut off the clustered buds and calluses at the base, and retain individual plants with a height greater than 2 cm, with a single stem of 1 cm Remove the leaves attached to the stalks to obtain the rooting material A;
[0028] 2) Rooting culture in a bottle: Inoculate the rooting material A into the rooting medium M in the bottle, and cultivate it under sealed aseptic conditions for 35 days to obtain the rooting bottle seedling B; wherein, the culture conditions are: light culture and dark culture every 12h Alternate cultivation, the cultivation temperature is 24°C, and the light intensity is 2000Lx;
[0029] The rooting medium M in the bottle is:...
Embodiment 2-6
[0035] Embodiment 2-6 is respectively a kind of rooting culture and seedling raising method of Ulmus chinensis tissue culture seedlings, and its steps are similar to embodiment 1, and the only difference is that the technical parameters involved therein are different, see the following table for details:
[0036]
[0037] The coagulant used in the above table is 20-40 mesh vermiculite.
[0038] The composition of the improved DKW basal medium involved in embodiment 2-6 is as follows:
[0039]
[0040] The Ulmus chinensis tissue culture seedlings prepared by the method of the above-mentioned embodiments 2-6 have more fibrous roots, strong root system vitality, dark green plant leaves, and faster growth. It can also be directly planted without the seedling washing process, saving labor costs, and is more suitable produced on an industrial scale. The rooting rate of the bred Ulmus chinensis tissue culture seedlings can reach more than 95.3%, and the survival rate can reach...
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