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A kind of rooting culture and seedling raising method of Thorn elm tissue culture seedling

A technology of rooting culture and rooting medium, which is applied in the field of rooting culture and seedling cultivation of Ulmus chinensis tissue culture seedlings, which can solve the problems of slow seedling growth, few elm thorns, and low reproduction coefficient, and achieve the improvement of cultivation survival rate and the degree of lignification Improve the effect of dark green leaves of plants

Active Publication Date: 2021-04-06
上海菁艺生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, seed propagation is prone to trait variation, which is not conducive to maintaining the excellent traits of the female parent; and the traditional asexual reproduction method, the female parent material cannot be obtained in large quantities, so the reproduction coefficient is low, the seedlings are slow, and a large number of seedlings cannot be provided, which limits its application.
[0004] At present, there are few research reports on Ulmus chinensis, only a small amount of research on traditional seedling cultivation, afforestation, biological characteristics, active substances, etc. There are almost no relevant reports on the research on tissue culture and rooting of Ulmus chinensis

Method used

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  • A kind of rooting culture and seedling raising method of Thorn elm tissue culture seedling
  • A kind of rooting culture and seedling raising method of Thorn elm tissue culture seedling
  • A kind of rooting culture and seedling raising method of Thorn elm tissue culture seedling

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Embodiment 1

[0026] A method for rooting culture and seedling raising of Ulmus chinensis tissue culture seedlings is characterized in that it comprises the following steps:

[0027] 1) Selection of rooting materials: select the subcultured tissue-cultured seedlings of Ulmus chinensis with a plant height of 3 cm and a subculture period of 30 days, cut off the clustered buds and calluses at the base, and retain individual plants with a height greater than 2 cm, with a single stem of 1 cm Remove the leaves attached to the stalks to obtain the rooting material A;

[0028] 2) Rooting culture in a bottle: Inoculate the rooting material A into the rooting medium M in the bottle, and cultivate it under sealed aseptic conditions for 35 days to obtain the rooting bottle seedling B; wherein, the culture conditions are: light culture and dark culture every 12h Alternate cultivation, the cultivation temperature is 24°C, and the light intensity is 2000Lx;

[0029] The rooting medium M in the bottle is:...

Embodiment 2-6

[0035] Embodiment 2-6 is respectively a kind of rooting culture and seedling raising method of Ulmus chinensis tissue culture seedlings, and its steps are similar to embodiment 1, and the only difference is that the technical parameters involved therein are different, see the following table for details:

[0036]

[0037] The coagulant used in the above table is 20-40 mesh vermiculite.

[0038] The composition of the improved DKW basal medium involved in embodiment 2-6 is as follows:

[0039]

[0040] The Ulmus chinensis tissue culture seedlings prepared by the method of the above-mentioned embodiments 2-6 have more fibrous roots, strong root system vitality, dark green plant leaves, and faster growth. It can also be directly planted without the seedling washing process, saving labor costs, and is more suitable produced on an industrial scale. The rooting rate of the bred Ulmus chinensis tissue culture seedlings can reach more than 95.3%, and the survival rate can reach...

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Abstract

The invention discloses a method for rooting culture and seedling raising of Ulmus chinensis tissue culture seedlings, comprising the following steps: 1) selecting rooting materials; 2) rooting culture in a bottle; 3) hardening seedlings in a bottle; 4) tending seedlings in a nutrient pot, Ulmus chinensis seedlings were obtained; the method solved the problem of low seedling emergence rate of Ulmus chinensis by traditional sowing, cutting and grafting propagation, and the tissue cultured seedlings of Ulmus chinensis cultivated had more fibrous roots, strong root system vitality, dark green leaves and faster growth. It is fast, and can be directly planted without the washing process of general tissue culture seedlings, which saves labor costs and is more suitable for industrial scale production. The rooting rate of the cultivated tissue culture seedlings can reach more than 95.3%, and the survival rate can reach more than 96.9%. ; The present invention is applicable to the seedling raising of elm thorn tissue culture seedlings.

Description

technical field [0001] The invention belongs to the field of plant cultivation, and relates to a method for rooting and seedling cultivation, in particular to a method for rooting cultivation and seedling cultivation of elm erythematosus tissue culture seedlings. Background technique [0002] Ulmus is a small deciduous tree of the family Ulmaceae, with a height of up to 10 meters, or in the shape of a shrub; it often grows in secondary forests on slopes below 2,000 meters above sea level; it is resistant to barrenness, drought, low temperature, and sandstorms, and is suitable for growth on various soils. It has strong adaptability, strong tillering ability, and slow growth rate. It can grow in areas with poor site conditions such as barren hills, barren slopes, and sandy land. Its role can be used firstly as a greening tree species for sand prevention and control, playing the role of windbreak and sand fixation, water and soil conservation, and water conservation; secondly, ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01H4/00
CPCA01H4/001A01H4/005A01H4/008
Inventor 冒文娟张玉杰林大为张承妹苏涌
Owner 上海菁艺生物科技有限公司
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