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Application of total glucosides of paeony in the preparation of drugs for treating thyroid cancer and improving the synergistic effect of tumor radiotherapy

A technology of total glucosides of paeony and thyroid cancer, applied in the field of traditional Chinese medicine, can solve the problems of ineffective treatment and decreased NIS expression level, and achieve the effect of improving radiotherapy sensitivity and enhancing anti-thyroid cancer.

Active Publication Date: 2021-11-05
ZHEJIANG CANCER HOSPITAL
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

RAIR-DTC is mainly due to the decrease of NIS expression level or ectopic expression outside the basement membrane, resulting in 131 I treatment does not work

Method used

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  • Application of total glucosides of paeony in the preparation of drugs for treating thyroid cancer and improving the synergistic effect of tumor radiotherapy
  • Application of total glucosides of paeony in the preparation of drugs for treating thyroid cancer and improving the synergistic effect of tumor radiotherapy
  • Application of total glucosides of paeony in the preparation of drugs for treating thyroid cancer and improving the synergistic effect of tumor radiotherapy

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0037] The preparation method of total glucosides of paeony, the specific process is: take appropriate amount of Radix Paeoniae Alba, add 70% ethanol respectively, 10 times the amount, extract twice, each time for 2 hours. Concentrate the medicinal liquid until 1 mL of medicinal liquid contains 1 g of crude drug, and use HPD300 macroporous resin to purify bran-fried total glucosides of paeony through investigation. It is determined that the medicinal liquid is diluted with water to contain 0.1 g of crude drug per 1 mL of medicinal liquid, and the flow rate of the sample is 4BV / h passes through the HPD300 macroporous adsorption resin column (resin column diameter-to-height ratio is 1:11), washes with water for 5 times column volume first, then elutes with 50% ethanol for 7 times column volume, and the elution flow rate is 4BV / h, collects and washes Remove liquid, recover ethanol under reduced pressure, filter, add 3% gelatin solution to the filtrate until no precipitation occur...

Embodiment 2

[0038] The pharmacological experiment of embodiment 2 total glucosides of paeony

[0039] 1. Experimental grouping

[0040] Cell and molecular biology experiments: Add the total glucosides of paeony prepared by the above method for 72 hours, the concentration of total glucosides of paeony is: 0.3mg / ml, 0.6mg / ml, 0.9mg / ml, 1.2mg / ml (Normal, IHH4, B-CPAP);

[0041]0.5mg / ml, 1mg / ml, 1.5mg / ml, 2.0mg / ml (8505c).

[0042] 2. IC50 screening drug concentration

[0043] 1) Seeding plate: inoculate cell suspension (200 μl / well) in a 96-well plate, and experiment with about 5000 cells per well.

[0044] 2) After 24 hours, wash with PBS 3 times.

[0045] 3) Drug treatment: according to the needs of the experiment, culture and stimulate with 200 μl of a specific concentration of drug solution for an appropriate period of time (for example: 24 hours).

[0046] 4) Wash with PBS 3 times.

[0047] 5) Add 200 μl of CCK-8 solution to each well. The wells with the corresponding amount of c...

Embodiment 3

[0140] Example 3 Total glucosides of paeony extract capsules or other health products

[0141] The composition of total glucosides of paeony extract capsules is

[0142] Element Dosage per tablet Total glucosides of paeony extract 100mg microcrystalline cellulose 35mg Micropowder silica gel 15mg stearic acid 15mg lactose 12mg

[0143] Add total glucosides of paeony (prepared according to the method of Example 1) into microcrystalline cellulose, micropowder silica gel, stearic acid, and lactose according to the prescription amount, mix evenly, and after making granules, fill them into No. 0 hard capsule shells and polish them. It is prepared (each capsule contains more than 80 mg of total glucosides of paeony). The recommended oral dosage for adults is 2-4 capsules / time, 2-3 times a day.

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Abstract

The invention relates to a new application of traditional Chinese medicine, in particular to the application of total glucosides of paeony in the preparation of medicines for treating thyroid cancer and improving the synergistic effect of tumor radiotherapy, belonging to the technical field of traditional Chinese medicine. Application of total glucosides of paeony in the preparation of medicines for treating thyroid cancer and improving tumor radiotherapy synergy. The total glucosides of paeony of the present invention can be used as an active ingredient, added to pharmaceutical excipients or carriers acceptable in pharmacy, and prepared according to the method recorded in pharmacy. The total glucosides of paeony of the present invention can also be used as one of the active ingredients, and also contain other drugs, add pharmaceutically acceptable pharmaceutical excipients or carriers, and make preparations according to the methods recorded in pharmacy. Through research, the inventors found that total glucosides of paeony have significant anti-thyroid cancer and enhanced iodine uptake function of cancer cells, can improve radiotherapy sensitivity, and thus can prepare drugs for treating thyroid cancer, as well as drugs for enhancing radiotherapy efficacy of tumors.

Description

technical field [0001] The invention relates to a new application of traditional Chinese medicine, in particular to the application of total glucosides of paeony in the preparation of medicines for treating thyroid cancer and improving the synergistic effect of tumor radiotherapy, belonging to the technical field of traditional Chinese medicine. Background technique [0002] Thyroid cancer (Thyroid cancer TC) is the most common endocrine malignancy, and has become a research hotspot due to the rapid increase in incidence in recent years. Papillary thyroid cancer (PTC) and follicular thyroid cancer (FTC) account for more than 90% of thyroid cancers, because they retain the functions of thyroid follicular cells to a certain extent, such as sodium iodide transport The expression of sodium iodide symporter (NIS), the ability to take iodine, the ability to secrete thyroglobulin (Tg), and the way of growth dependent on thyroid stimulating hormone (thyrotropin, TSH), etc., are call...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K36/71A61K36/65A61P35/00
CPCA61K36/65A61K36/71A61K2236/333A61K2236/39A61K2236/51A61K2236/55A61P35/00
Inventor 李清林葛明华郑传铭
Owner ZHEJIANG CANCER HOSPITAL
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