Screening method of heat-resistant chrysanthemum

Abstract

The invention discloses a screening method for heat-resistant chrysanthemums. The method comprises the following steps: treating chrysanthemums with high-temperature stress at the seedling stage, observing and statistically detecting the severity of high-temperature injury symptoms, seedling survival rate, oxidation The degree of damage and the expression level of heat-shock-related genes; treat chrysanthemums with high-temperature stress during the flowering stage, and observe and statistically detect the severity of high-temperature damage symptoms, whether they can bloom, and the level of related physiological indicators; cultivate chrysanthemum seedlings In the field of tropical areas, the physiological and biochemical indicators are measured, and the flowering rate and flowering period of each variety are observed and counted; the results of all indicators in the three steps are combined to screen out heat-resistant chrysanthemum varieties. The invention combines high-temperature stress treatment and field cultivation test, and is an effective way to efficiently and comprehensively obtain heat-resistant varieties (lines). This will be a good research method for introducing and cultivating heat-resistant chrysanthemum varieties in southern high-temperature regions.

Description

technical field [0001] The invention relates to a method for screening plant varieties, in particular to a method for screening heat-resistant chrysanthemums. Background technique [0002] Chrysanthemum (Chrysanthemum morifolium) is a perennial herbaceous perennial flower of the genus Chrysanthemum in the family Asteraceae. It is one of the top ten traditional famous flowers in China, one of the top four cut flowers and top ten potted flowers in the world. It has extremely high ornamental and application value, and plays a very important role in flower production. status. [0003] Chrysanthemums like mild and cool climates, and the optimum temperature for growth and development is between 15 and 25°C. High temperatures above 25°C are not conducive to the growth of chrysanthemums. It has been reported that chrysanthemum grows slowly when the temperature is above 32°C, and can withstand high temperatures of 40°C in summer, but basically stops growing. The high temperature in...

AI Technical Summary

Problems solved by technology

However, it takes too long to obtain transgenic plants, and the difficulty of transgenic is related to the variety. At present, the transgenic technology of chrysanthemums is not mature enough, and it is still difficult to successfully transfer genes into chrysanthemum varieties.

[0006] Most of the previous studies were to observe the heat resistance comparison of experimental chrysanthemum varieties under simulated high temperature. However, on the one hand, the direct identification of artificial simulation will be limited by equipment factors, and it cannot completely simulate all natural conditions and the amount of experimental materials is limited.

On the other hand, the field test results are easily affected by the location, and the reliability of the local test results is small

[0007] So far, no relevant reports have been found on the introduction of chrysanthemums and the selection of heat-resistant chrysanthemum varieties in tropical regions

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