Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Special preparation of high-resolution chromosome staining solution for karyotype analysis

A karyotype analysis and high-resolution technology, applied in the biological field, can solve the problems of unsatisfactory effect, poor staining texture, long staining time, etc., and achieve the effects of improving uniformity and clarity, clear staining effect, and improving staining quality

Active Publication Date: 2021-10-29
BEIJING INST OF HUMAN GENETICS & REPRODUCTION MEDICINE LTD CHINA +3
View PDF10 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] At present, the Giemsa reagents of various manufacturers have unsatisfactory results when doing high-resolution and super-high-resolution karyotypes. The performance is: long staining time, uneven banding, and poor staining texture.
Because the preparation of the existing G banding dye solution is not standardized enough, the banding is blurred, which affects the appearance of the banding, such as patents CN 103710435 B and CN 103308361 B
This brings difficulties to the analysis of chromosome karyotype results, affecting the quality of chromosome preparation and the external quality assessment of karyotype analysis results

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Special preparation of high-resolution chromosome staining solution for karyotype analysis
  • Special preparation of high-resolution chromosome staining solution for karyotype analysis
  • Special preparation of high-resolution chromosome staining solution for karyotype analysis

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0043] The preparation method of the special high-resolution chromosome dye solution for karyotype analysis of the present invention:

[0044] 1. Dye solution formula

[0045] Giemsa powder 1g, glycerin 66ml, methanol 66ml.

[0046] 2. Preparation method

[0047] (1) Mix 1 g of Giemsa powder and 6 ml of glycerin, and grind continuously for 2 hours in the dark.

[0048] (2) Transfer the Giemsa grinding mixture into a 100ml brown bottle, and add 60ml of glycerin. Then incubate at 58°C in the dark for 2 hours.

[0049] (3) Then transfer to a 500ml brown bottle, add 66ml methanol and mix well. After being sealed and protected from light and stored at room temperature for 2 weeks, the performance test of Giemsa dye solution can be carried out.

[0050] (4) Dye liquor performance test: use G-belt dyeing technology to test the dye liquor performance:

[0051] A. Cylinder No. 1: Put the baked human peripheral blood sample slides into the trypsin mixture and digest at 37°C;

[0...

Embodiment 2

[0068] The preparation method of the special high-resolution chromosome dye solution for karyotype analysis of the present invention:

[0069] 1. Dye solution formula

[0070] Giemsa powder 1g, glycerin 66ml, methanol 66ml.

[0071] 2. Preparation method

[0072] (1) Mix 1 g of Giemsa powder and 6 ml of glycerin, and grind continuously for 2 hours in the dark.

[0073] (2) Testing indicators: After grinding, the next step will start only after the particle size of the Giemsa dyeing powder has been tested twice and passed. Specific steps are as follows:

[0074] A. Manual detection step 1: Take 10 μl of Giemsa grinding mixture and apply it on the glove and twist it. If the particles are visible to the naked eye, and if the particles are touched by finger twisting, the grinding is not sufficient. Continue grinding in step (1) until it cannot be touched. until particles are present.

[0075] B. Microscopic detection step 2: After no particles can be found by twirling, under ...

Embodiment 3

[0088] The preparation method of the special high-resolution chromosome dye solution for karyotype analysis of the present invention:

[0089] 1. Dye solution formula

[0090] Giemsa powder 1g, glycerin 66ml, methanol 66ml, dispersant.

[0091] Wherein, described dispersant:

[0092] The dispersant of experimental group 1 is ethylene oxide with a working volume of 1ml.

[0093] The dispersant of experimental group 2 is ethylene oxide with a working volume of 2.5ml.

[0094] The dispersant of experimental group 3 is ethylene oxide with a working volume of 5ml.

[0095] The dispersant of experimental group 4 is ethylene oxide with a working volume of 10ml.

[0096] The dispersant of comparative group 1 is ethylene oxide with a working volume of 0ml.

[0097] 2. Preparation method

[0098] (1) Mix 1 g of Giemsa powder and 6 ml of glycerin, and grind continuously for 2 hours in the dark.

[0099] (2) Testing indicators: After grinding, the next step will start only after th...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The present invention relates to special preparation of high-resolution chromosome dye solution for karyotype analysis, which is prepared from the following raw materials: 1-3g of Giemsa dye powder, 66-69ml of glycerin, 66ml of methanol, and 1-10ml of dispersant; The invention makes the dye particles finer and standardized by grinding the dye particles, and improves the dispersion characteristics of the dye particles by adding a dispersant, thereby greatly improving the uniformity and clarity of dyeing. The novel dye solution of the invention obviously improves the dyeing quality of high-resolution chromosome banding. The staining effect on high-resolution chromosomes is clearer, and the staining speed on human chromosomes is fast, controllable, and the bands are clear. It can be applied to large-scale clinical genetic laboratories.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to the special preparation of high-resolution chromosome dye solution for karyotype analysis. Background technique [0002] Chromosomes are the carriers of genes. Chromosomes may undergo quantitative and structural changes, which may cause various genetic defects, congenital genetic diseases, embryo cessation, spontaneous abortion, tumors, etc. Diseases caused by chromosomal abnormalities are generally called chromosomal diseases. [0003] Chromosomal detection is currently mostly used in clinical practice such as reproduction, obstetrics and gynecology, newborns, blood diseases, tumors and other specialties. Genetic diagnosis using the technique of chromosome banding after cell culture is relatively simple and reliable, and it is still the gold standard test method. [0004] Chromosomal karyotype analysis is mainly divided into several steps: cell culture, harvest, preparation, bandi...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Patents(China)
IPC IPC(8): G01N1/30
CPCG01N1/30
Inventor 李存玺刘恒涛刘舒李能干
Owner BEIJING INST OF HUMAN GENETICS & REPRODUCTION MEDICINE LTD CHINA
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com