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A method to improve the accuracy of basic data for protein content modeling by near-infrared spectroscopy

An accuracy and data technology, which is applied in the direction of material analysis, analysis materials, and measuring devices by optical means, can solve the problems of great influence on accuracy, and achieve the effect of improving data detection accuracy and fast detection speed.

Active Publication Date: 2021-03-12
SHANDONG UNIV
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Problems solved by technology

At the same time, NIRS also has certain limitations, mainly reflected in the fact that NIRS is a secondary analysis method, which requires traditional analysis methods to measure basic data, and its accuracy is greatly affected by the reference method

Method used

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  • A method to improve the accuracy of basic data for protein content modeling by near-infrared spectroscopy
  • A method to improve the accuracy of basic data for protein content modeling by near-infrared spectroscopy
  • A method to improve the accuracy of basic data for protein content modeling by near-infrared spectroscopy

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Embodiment 1

[0031] 1. Error analysis of human plasma reference substance measured by biuret method and Kjeldahl method

[0032] Raw material human plasma reference material (Shandong Taibang Biological Products Co., Ltd., China, protein content value = 58.1), using the Kjeldahl method and the biuret method (AU5800 automatic biochemical analyzer, Beckman, the United States) in parallel Measure 20 times and calculate the average value of the measurement error. Utilize Kjeldahl method and biuret method to measure the result of raw material human plasma reference substance as follows figure 1 shown. The average measurement errors of the biuret method and the Kjeldahl method are 1.676g / L and 0.672g / L respectively, that is, the accuracy of the Kjeldahl method is better than that of the biuret method. Therefore, it is proposed that for the biuret method with larger measurement errors, if you want to obtain a stable and accurate cumulative average value, you should increase the measurement data...

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Abstract

The invention belongs to the field of protein content detection, and specifically relates to a method for improving the accuracy of basic data in near-infrared spectroscopy analysis technology, that is, protein detection data, based on the idea of ​​"data averaging". For the detection of protein content in the prior art, the method of averaging three measurements is usually adopted to improve the accuracy of the data. The present invention applies the idea of ​​"value averaging" to the problem of protein content detection, obtains the optimal measurement times for protein content measurement, and averages multiple measurements to improve the accuracy of protein detection data. The basic data detection applied to the modeling of human plasma protein content by near-infrared spectroscopy analysis technology, the number of parallel measurements of the basic data should be 39 times. The near-infrared spectrum analysis model established by the method is increased by 23.28% compared with the RMSEP modeled by repeating three parallel measurements to obtain an average model. The method of the invention is applied to the detection of plasma proteins and has great significance.

Description

technical field [0001] The invention belongs to the field of protein content detection, and specifically relates to a method for improving the accuracy of basic data in near-infrared spectroscopy analysis technology, that is, protein detection data, based on the idea of ​​"data averaging". Background technique [0002] Biuret (NH 2 CONH CONH 2 ) is the product obtained after two molecules of urea are heated at about 180°C to release one molecule of ammonia. Any compound with two amide groups or two directly connected peptide bonds, or a peptide bond that can be connected by an intermediate carbon atom, can undergo biuret reaction. In strong alkaline solution, biuret forms a purple complex with divalent copper ions. The color of the purple complex is proportional to the protein concentration and has nothing to do with the protein molecular weight and amino acid composition, so it can be used to determine the protein content. The biuret method has similar color reactions to...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N21/359
CPCG01N21/359
Inventor 臧恒昌孙钟毓聂磊李丹阳井金荣杜冉冉刘瑞琛于宸
Owner SHANDONG UNIV
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