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A biocontrol strain pnc25 for controlling litchi frost blight and its application

A technology of biocontrol strains, Phytophthora litchii, applied in the fields of application, bacteria, biocide, etc., can solve the problems that have not yet been seen such as the function of Microbacterium acetylides in preventing and controlling Lychee frost blight, and achieve excellent biocontrol effects, low requirements for cultivation conditions, and The effect of good fresh-keeping effect

Active Publication Date: 2021-07-27
中国热带农业科学院广州实验站
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the effective measures for the control of litchi frost blight are mainly chemical control. However, due to the environmental pollution caused by the extensive use of chemical pesticides and the problem of drug resistance of pathogens, the research and development of low-toxic, non-toxic The use of residual biogenic pesticides in the prevention and control of litchi diseases is of great significance for controlling the amount of pesticide residues, ensuring food safety and improving the international competitiveness of products
So far, there are no related reports about the function of Microbacterium acetylides in preventing and controlling frost blight of litchi

Method used

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  • A biocontrol strain pnc25 for controlling litchi frost blight and its application
  • A biocontrol strain pnc25 for controlling litchi frost blight and its application
  • A biocontrol strain pnc25 for controlling litchi frost blight and its application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] Strain source: PNC25 was isolated from the pulp of "Yuannuo" in a lychee orchard in Huilai, Jieyang City, Guangdong Province.

[0027] The collected samples were quickly packed into numbered plastic bags and brought back to the laboratory for separation. Cut 3 g of pulp, soak it in 1 mL of 1% sodium hypochlorite for 5 minutes, soak it in 70% alcohol for 2 minutes, and wash it three times with sterile water. (Take 0.1mL of the last cleaning solution and apply R2A, or take 0.1mL of the last cleaning solution and add R2A 2 A culture medium, shake cultured at 30°C, and if it grows sterile after 48h, the surface is considered to be clean). Grind the surface-sterilized pulp with a sterilized pestle, add 3 mL of sterile water, filter with sterile cotton cloth, dilute 10 times with sterile water, take 0.1 mL and dilute in turn, take 10 -4 , 10 -5 , 10 -6 Three gradients of 0.1mL coated with R 2 Plate A, incubate at 28°C for 48h, select a plate with a colony number between ...

Embodiment 2

[0036] Example 2 Preparation of inoculum

[0037] (1) Cultivation of seed bacterial liquid: inoculate PNC25 (GDMCC No.60108) into LB medium, cultivate at 28°C and 200rpm with shaking for 24h, and after 16h, take samples every 3h in the ultra-clean workbench to measure the OD value at 600nm , in the measurement process, the culture solution without bacteria inoculation is used to zero, until the OD value of the seed bacteria solution is between 0.5 and 0.8, the culture is ended, and the seed bacteria solution is obtained;

[0038] (2) Preparation of wet bacteria: add the seed bacteria solution into the LB culture solution at a volume ratio of 1:500, and shake and culture at 28°C and 200 rpm for 24 hours to obtain a biocontrol agent with a viable bacteria concentration of 1 × 10 8 ~10 9 CFU / mL.

Embodiment 3

[0039] Example 3 Evaluation of the indoor (LT, laboratory trial) disease control effect of biocontrol bacteria PNC25 on litchi fruit / leaf

[0040] The healthy lychees picked in the field are processed as follows: A. The biocontrol bacteria stock solution is diluted to a final concentration of 5*10 7 CFU / mL, spray fruit / dilute to final concentration 1*10 7 CFU / mL sprayed leaves; B. LB solution at the same dilution. Three replicates were set for each treatment, each replicate was 30 fruits / 5 branches, and the fruits / twigs were placed in a fresh-keeping box (the bottom of the box was covered with sterilized filter paper, 18cm in diameter, moistened with sterilized water, and the size of the fresh-keeping box was 323*220 *100mm, the manufacturer is Hualong Plastic Factory, Chancheng District, Foshan). Each treatment (3 replicates) of bacterial liquid and control group was sprayed with a total of 300 mL. 24hpt (hours post treatment) spray inoculation with pathogenic bacteria P.lit...

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Abstract

The invention discloses a biocontrol bacterial strain PNC25 for preventing and treating litchi frost blight and its application. A biocontrol strain PNC25 for the control of litchi frost blight, Exiguobacterium acetylicum, was preserved in Guangdong Microbial Culture Collection Center on November 15, 2016, with the strain preservation number GDMCC No.60108. The acetyl microbacterium of the present invention has inhibitory effect on litchi frost blight, and has excellent biocontrol effects in preventing and treating litchi frost blight under multiple experimental conditions. The biocontrol agent of the present invention can be used in the prevention and treatment of litchi frost blight . At the same time, the microbacterium acetylides of the invention has a good fresh-keeping effect on litchi and can be used to prepare litchi fresh-keeping preparations.

Description

technical field [0001] The invention belongs to the technical field of plant protection, and relates to a biocontrol strain PNC25 for preventing and treating litchi frost blight and its application. Background technique [0002] Litchi (Litchi chinensis Sonn) is an important subtropical economic crop in the southern region. It is known as "the precious fruit of China". It has high economic value and is one of the most competitive fruits in my country in the international market. However, in the main litchi producing areas of my country, Phytophthora downy mildew caused by Peronophythora litchii Chen ex Ko et al. is one of the most important diseases causing litchi yield reduction. The epidemic of the disease has the characteristics of indirect outbreak, which is greatly affected by the climate. In suitable years for the disease, it can cause more than 80% of the loss of lychees. At present, the effective measures for the control of litchi frost blight are mainly chemical co...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20A01N63/20A01P3/00C12R1/01
CPCA01N63/00C12N1/20C12N1/205C12R2001/01
Inventor 郑丽吴健雄蒋仁娇
Owner 中国热带农业科学院广州实验站
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